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SUSCEPTIBILITY OF PINK BOLLWORM, PECTINOPHORA GOSSYPIELLA (SAUNDERS) TO BACILLUS THURINGIENSIS BERLINER CRY TOXINS

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Title SUSCEPTIBILITY OF PINK BOLLWORM, PECTINOPHORA GOSSYPIELLA (SAUNDERS) TO BACILLUS THURINGIENSIS BERLINER CRY TOXINS
Ph D
 
Creator SANYASI DHURUA
 
Contributor G.T.Gujar
 
Subject productivity, millets, biological phenomena, value added product, participation, economic systems, markets, land resources, mass media, technological changes
 
Description T-8321
The pink bollworm [Pectinophora gossypiella (Saunders)] infestation was studied in
the cotton bolls collected from 15 locations in the country and found to vary from 0 to 66.3%
in terms of locule damage and to the maximum of 2.1 larvae per boll. Of these, six
populations from five locations were successfully reared on an artificial diet and bioassayed
for their susceptibility to Bacillus thuringiensis (Bt) derived Cry1Ac and the mixture of
Cry1Ac and Cry2Ab as expressed in the seeds of Bollgard II cotton through diet
incorporation bioassays. Sixteen day median lethal concentrations (LC50) of Cry1Ac did not
vary widely for 5-day old larvae of P. gossypiella except Amreli population (1.57 µg/ml)
which was 42 fold resistant than the most susceptible Adilabad population (0.037 µg/ml).
Further, susceptibility of the Amreli population was at par with those of others to mixture of
Cry1Ac and Cry2Ab toxins expressed in Bollgard II hybrid KDCHH441BGII, suggesting the
synergistic toxicity and absence of cross resistance. These baseline susceptibility data for P.
gossypiella to Cry1Ac and the mixture of Cry1Ac and Cry2Ab can be used as benchmark for
monitoring resistance to Bollgard (and other Cry1Ac based Bt cotton events) and Bollgard II
which are planted over 80% of cotton area in India.
 
Date 2016-10-22T09:24:24Z
2016-10-22T09:24:24Z
2010
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/81331
 
Format application/pdf
 
Publisher IARI,DIVISION OF ENTOMOLOGY