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Molecular marker assisted introgression of null allele of kunitz trypsin inhibitor (ti) in soybean (Glycine max L.)

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Title Molecular marker assisted introgression of null allele of kunitz trypsin inhibitor (ti) in soybean (Glycine max L.)
Ph.D.
 
Creator SHIVAKUMAR M.
 
Contributor A. Talukdar
 
Subject Soybean, kunitz trypsin inhibitor, biochemical screening, residual activity and TI locus
 
Description T-8772
Kunitz trypsin inhibitor (KTI), a major anti-nutritional factor present in soybean seed, is
a major bottleneck in industrial use of soybean and public acceptance of soybean food
products. Biochemical screening was carried out for analyzing trypsin inhibitor contents
in 145 genotypes comprising exotic collections and released varieties. The content of
trypsin inhibitor ranged from 14.15 to 186.27 mg g-1 of seed meal. Lowest level of
trypsin inhibitor content was observed in genotype PI542044, (14.15 mg g-1 seed meal).
Popular Indian soybean varieties viz., JS335, JS9305, NRC37, NRC7, PK1225, DS9712,
JS9752, and DS9814 contained higher level of trypsin inhibitor. Heat treatment could
reduce trypsin inhibitor activity partially leaving about 20% as residual activity. The
study indicated the need for other ways like site directed mutagenesis to create
genotypes with reduced KTI for use in breeding program.
 
Date 2016-08-22T14:43:54Z
2016-08-22T14:43:54Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/73314
 
Format application/pdf
 
Publisher IARI, Division of Genetics