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GENETICS AND MOLECULAR CHARACTERIZATION OF MALE FERTILITY RESTORATION AND IDENTIFICATION OF STABLE MALE FERTILITY RESTORERS IN PIGEONPEA (Cajanus cajan (L.) Millsp.)

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Title GENETICS AND MOLECULAR CHARACTERIZATION OF MALE FERTILITY RESTORATION AND IDENTIFICATION OF STABLE MALE FERTILITY RESTORERS IN PIGEONPEA (Cajanus cajan (L.) Millsp.)
 
Creator RAGHU, B. R.
 
Contributor R.S. Raje
 
Subject drying, nut products, dryers, coconuts, energy resources, biological phenomena, crop residues, irrigation, fats, proteins
 
Description T-8566
Pigeonpea (Cajanus cajan (L.) Millsp.), an economically and
nutritionally important grain legume. Although, information on the genetics of male
fertility restoration is available in pigeonpea but no study reported inheritance
studies on the male fertility restoration for A2 cytoplasm. In the present study, seven
crosses were studied to generate information on the genetics of male fertility
restoration of the A2 cytoplasm. On the basis of results obtained it was found that
two complementary genes controlling the male fertility restoration in three crosses,
in which all three restorers were derived from single cross. Whereas, in remaining
four crosses the male fertility restoration was governed by the single dominant gene.
It was observed that there was no influence of genetic background on male fertility
restoration. In order to isolate stable male fertility restores, seven hybrids were tested
at three locations each over two years for percent male fertility and all hybrids were
found to be stable in expression for male fertility.
As, there is no molecular diversity information available on parental lines
of A2 cytoplasm based hybrids. This study deals with the use of 390 EST-SSR
markers on 8 A lines (Cytoplasmic genetic male sterile) (2 are A4 cytoplasm based
and 6 are A2 cytoplasm based), 2 B lines (Maintainers) and 19 R lines (restorer
lines). In total, 60 markers (15.3%) showed polymorphism with 2 to 4 (average 2.26)
alleles and 0.1780 to 0.5885 (average of 0.358) polymorphic information content
(PIC) value per marker. The parental combinations of A and R lines viz., ICPA2047
X ICPR2740, ICPA2043 X ICPR3472, ICPA2043 X ICPR4013, MS10A X AKPR9,
MS10A X AKPR100 X GT288A X AKPR417 were identified as most distinct and
divergent. Therefore, identified hybrid parental combination could be effectively
used as candidate parental genotypes in hybrid development programme. ASSR236,
a SSR marker found to be linked to male fertility restorer gene at a distance of
19.3CM. Therefore, the information generated from the present investigation, will
helps to generate highly heterotic hybrid. and also, as parents in development of
mapping population and trait mapping.
Key words: Microsatellite markers, Inheritance, male fertility restoration,
pigeonpeam, Genetic diversity
 
Date 2016-12-14T10:24:35Z
2016-12-14T10:24:35Z
2012
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/90035
 
Format application/pdf
 
Publisher IARI, DIVISION OF GENETICS