Record Details

Plant regeneration studies in guar patta (Aloe vera L.)

KrishiKosh

View Archive Info
 
 
Field Value
 
Title Plant regeneration studies in guar patta (Aloe vera L.)
 
Creator Saroha, Vandanna
 
Contributor Yadav, Neelam R.
 
Subject Aloe vera L., Micropropagation, TDZ, Paclobutrazol
 
Description The present study was conducted to develop micropropagation system in
Aloe vera L. and establishment of the regenerated plantlets in the soil. Shoot tip
and leaf explants from 4-5 cm long offshoots were collected from field grown
plants. These explants were cultured on 18 different MS modified media fortified
with different concentrations and combinations of auxin, cytokinins and additives.
MS medium supplemented with TDZ (1.0 mg/l) produced highest shoot formation
response and further upscaling was done on MS medium with BA (2.0 mg/l) and
IAA (0.2 mg/l).
For shoot elongation, Tyrosine (50.0 mg/l) alongwith BA (5.0 mg/l) and
adenine sulphate (80 mg/l) in MS medium produced 158.5 per cent increase in
shoot length along with rooting. Leaf explants did not respond to shoot formation,
however, 24.9 per cent callusing was observed on medium with 2,4-D (1.0 mg/l).
The regenerated shoots showed 100 per cent rooting in all the six media tried for
rooting, but longest roots (9.16 cm) and highest number of roots per shoot (13.22)
were observed on medium fortified with paclobutrazol (0.5 mg/l) after 45 days of
transfer. The regenerated plants were kept for hardening in water overnight and
transferred to the pots with soil and vermiculite mixture (1:1). The rooting
medium also affected the survival rate of the regenerated plants in soil. Highest
survival rate was observed in paclobutrazol rooted plantlets. The regenerated
plants were analysed for homogeneity through SDS-PAGE and all the plants
showed similar soluble protein banding pattern.
Promising variant has been recorded among plants regenerated on
paclobutrazol (0.5 mg/l) containing medium. It also contained around four times
more soluble protein and one and a half times more sapogenin content than the
field grown plants. It showed thin cuticle, succulent and broad leaves. These
plants are under observation for their utility in gel extraction.
 
Date 2016-11-30T09:14:55Z
2016-11-30T09:14:55Z
2005
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/88287
 
Language en
 
Format application/pdf
 
Publisher CCSHAU