Screening and characterization of Mesorhizobium ciceri isolates for antagonistic effect to Fusarium oxysporum f. sp. ciceri
KrishiKosh
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Title |
Screening and characterization of Mesorhizobium ciceri isolates for antagonistic effect to Fusarium oxysporum f. sp. ciceri
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Creator |
Suman
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Contributor |
Yadav, A.S.
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Subject |
Acidity, Guavas, Papayas, Storage, Fruits, Sugar, Reducing sugars, Beverages, Vitamins, Biological phenomena
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Description |
Screening and characterization of Mesorhizobium ciceri isolates for antagonistic effect to Fusarium oxysporum f. sp. ciceriā was investigated on 207 isolates obtained from the nodules of chickpea plant. The growth of these 207 isolates was checked on YEMA Congo red medium. Out of 207 isolates, 117 were fast growing and 90 were slow growing rhizobia. These 207 isolates were screened for their anagonistic effect against Fusarium oxysporum. Out of 207 isolates, seven isolates were found to be antagonistic to F. oxysporum. Size of inhibition zone varied among the seven isolates from 5 mm to 15 mm. MC99 produced the largest inhibition zone. These seven isolates were further studied for antibiotic, siderophore, indole acetic acid (IAA) production and phosphate solubilization. None of the seven isolates was producing antibiotic. Out of these seven isolates, MC84, MC96, MC99 were producing siderophore and MC69, MC180, MC183 and MC190 were not producing siderophore. So, the mechanism of antagonism might be different in all these isolates. All the seven isolates were produing IAA, which varied among these isolates. The isolate MC99 was producing maximum indole acetic acid. None of the seven isolates was solubilizing tricalcium phosphate. In the present study, the isolate MC99 was found to be having maximum antagonistic activity against F. oxysporum, as it produced maximum zone of inhibition against this fungus. It had also the maximum amount of IAA and siderophore. |
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Date |
2016-12-16T11:43:22Z
2016-12-16T11:43:22Z 2010 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/90531
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Language |
en
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Format |
application/pdf
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Publisher |
CCSHAU
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