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Studies on genetic diversity in chickpea (Cicer arietinum L.) genotypes using morphological and molecular markers

KrishiKosh

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Title Studies on genetic diversity in chickpea (Cicer arietinum L.) genotypes using morphological and molecular markers
 
Creator Janghel, Dharmendra Kumar
 
Contributor Krishan Kumar
 
Subject Genotypes, Chickpeas, Planting, Sowing, Genetics, Developmental stages, Yields, Biological phenomena, Phenotypes, Cicer arietinum
 
Description The present investigation entitled “Studies on Genetic Diversity in Chickpea
(Cicer arietinum L.) Genotypes using Morphological and Molecular Markers,” was
carried out at the Research area of Pulses Section, Seed Testing Laboratory, Seed Science
and Technology Section and Post-Graduate (PG) Laboratory, Department of Genetics and
Plant Breeding, CCS Haryana Agricultural University, Hisar (Haryana, India) during Rabi
2014-15. The observations were recorded for different morphological characteristics both
qualitative and quantitative to assess the genetic diversity in 60 chickpea genotypes (both
Desi and Kabuli types). These genotypes were characterized and grouped into different
classes on the basis of observations recorded for 13 different qualitative characters as per
the guidelines for the conduct of test for Distinctiveness, Uniformity and Stability on
chickpea (Cicer arietinuin L.). The observations were also recorded on 11 yield and its
component traits for these genotypes to assess the genetic diversity. The GCV and PCV
were observed high for 100 seed weight, number of pods per plant, seed yield per plant and
number of secondary branches per plant. High heritability coupled with high genetic
advance for traits viz., number of pods per plant, seed yield per plant and 100 seed weight
indicated additive gene effects. Seed yield per plant had a positive and highly significant
correlation with number of secondary branches per plant, number of pods per plant, number
of seeds per pod, standard germination per cent, seedling length and seed vigour index-I.
The Non-hierarchical Euclidean cluster analysis grouped 60 chickpea genotypes into seven
clusters. The inter cluster distance was observed maximum (7.84) between cluster III and
cluster VII which showed that maximum genetic diversity is present between the genotypes
of these two clusters. Out of these 60 genotypes, 45 promising chickpea genotypes were
selected to study the genetic diversity using ISSR molecular markers. The genetic
fingerprinting of 45 chickpea genotypes using 25 ISSR primers showed polymorphism
information content (PIC) values from 0.66 (UBC -824) to 0.90 (UBC -812) with an average
of 0.80. Based on morphological and molecular genetic diversity studies, the genotypes viz.,
H 04-99, H 07-157, H08 -18, HC-3, HC-5, HK-1, HK-2, ICCV-2, ICCV-6, ICCV-10, JG-11,
DCP 92-3, JG-315, JGK-1 and JGK-27 showed sufficient amount of genetic diversity for
yield and its component traits which may be used in chickpea breeding programmes for
further improvement.
 
Date 2017-01-25T09:16:52Z
2017-01-25T09:16:52Z
2016
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/97494
 
Language en
 
Format application/pdf
 
Publisher CCSHAU