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Isolation and screening of biosurfactant producing bacteria from hydrocarbon polluted environment

KrishiKosh

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Title Isolation and screening of biosurfactant producing bacteria from hydrocarbon polluted environment
 
Creator Kavita Rani
 
Contributor Sangwan, Seema
 
Subject Planting, Sowing, Genotypes, Yields, Developmental stages, Cowpeas, Diseases, Genetics, Harvesting, Harvest index
 
Description Biosurfactants are surface active metabolites containing hydrophobic and hydrophilic moieties
that reduce surface and liquid-liquid or solid-liquid interfacial tensions. As biosurfactants have been
found potential candidates for many applications in various aspects, the development of this line of
research is of paramount importance. Isolation, screening and identification of biosurfactant producing
bacteria from hydrocarbon contaminated water and soil samples were assessed. Water sample collected
in the form of surface microlayer from pond, near library, CCS Haryana Agricultural University, Hisar
along with total five soil samples collected from petrol pumps and automarket, Hisar, were used for
retrieval of total 78 bacterial isolates by enrichment culture technique using crude oil, liquid paraffin
and diesel as the carbon source.
Only 16 isolates have shown haemolytic activity on blood agar medium which were further
screened by using different screening methods, using culture supernatant grown in MS broth. Highest
oil displacement was generated by BK68 (6.23cm) followed by BK23 (2.48cm), BK34 (2.23cm) and
BK48 (2.06cm). Highest E24 index was given by culture supernatant of BK66 (19.5%) followed by
BK68, BK58, BK23 (19.0%, each) and BK34 (18.0%) after 4 days of incubation. Isolate BK68,
however showed further increase in E24 value to 20.0% on 5th day of incubation. Highest reduction in
surface tension was given by BK68 (71dynes/cm), followed by BK23 (37dynes/cm), BK66
(36dynes/cm) and BK58 (33dynes/cm). Highest oil atomization was given by BK68 (0.50cm) followed
by BK23 (0.40cm), BK34 (0.38cm), BK66 (0.35cm), BK58 (0.30cm), BK48 (0.30cm) and BK5
(0.30cm).Based on the results of different screening methods, total six higher biosurfactant producing
bacterial isolates were selected for identification. Isolates BK23, BK58 and BK68 were found to be
aerobic, G+ve and endospore forming bacteria, therefore, may be identified as Bacillus spp. Isolate
BK66 being G+ve, coccus (in bunches), catalase +ve, and showing β-haemolysis may be identified as
Staphylococcus spp. Remaining two isolates BK34 and BK48 were G-ve, rods, oxidase +ve and
glucose fermentation –ve, therefore, may be identified as Pseudomonas spp. All the identified cultures
were subjected to biosurfactant production using MS broth containing 2% diesel as the carbon source
and highest production was reported by BK68 (18.0%) as evident from emulsification index.
 
Date 2016-10-19T09:52:59Z
2016-10-19T09:52:59Z
2015
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/80925
 
Language en
 
Format application/pdf
 
Publisher CCSHAU