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Molecular diversity analysis in drought and salt tolerant genotypes of Indian mustard, brassica junceaL. (Czern. and coss.)

KrishiKosh

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Title Molecular diversity analysis in drought and salt tolerant genotypes of Indian mustard, brassica junceaL. (Czern. and coss.)
 
Creator Saini, Prince
 
Contributor Yadav, N.R.
 
Subject Surface water, Irrigation, Water quality, Statistical methods, Liquid wastes, Sampling, Storage structures, Area, Wastes, Water
 
Description In the present study, 25 genotypes of Brassica juncea along with 5 related species varying for
drought and salt tolerance were selected for genetic diversity analysis using SRAP and EST-SSR
markers. DNA from young leaves of 30 genotypes of Brassica was isolated using CTAB method. Forty
combinations of SRAP primers and twenty three EST-SSR primers were used and the PCR reaction
conditions were standardized for both. The amplified product was separated using agarose gel
electrophoresis. Out of forty SRAP and twenty threeEST SSR primers used, only 37 and 18 primers
showed amplification respectively. In total, 423 bands were obtained, out of which 422 were
polymorphic. The average number of bands in SRAP was 8.25 and 4.4 in EST-SSR. Average
polymorphism across the 30 Brassica genotypes and related species was 99.78%. Based on similarity
matrix data and analysis by NTSYS-PC, the value of similarity coefficient ranged from 0.613 to 0.943
with an average of 0.76. Dendrogram was constructed using similarity matrix using UPGMA
subprogram which divided the 30 genotypes of Brassica and related species into five clusters. The
dendrogram divided the 30 Brassica genotypes into 5clusters with related species grouped together.
Two and three dimension principal component analysis (PCA) based the data was also obtained.
 
Date 2016-10-27T09:06:17Z
2016-10-27T09:06:17Z
2012
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/82381
 
Language en
 
Format application/pdf
 
Publisher CCSHAU