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Identification of Genes Associated with Mango Malformation through Differential Expression Analysis

KrishiKosh

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Title Identification of Genes Associated with Mango Malformation through Differential Expression Analysis
 
Creator ASHOK YADAV
 
Contributor K. Usha
 
Subject null
 
Description t-9785
Mango, the King of fruits belongs to the family Anacardiaceae and occupies a
paramount place among the fruit crops grown in India owing to its delicious flavour
and taste. Out of several diseases that attack mango crop, malformation caused by
Fusarium mangiferae is a serious threat to mango cultivation in various countries, since
it causes 50-90% yield losses. Differentially expressed genes (DEGs) in healthy and
malformed buds of mango variety Amrapali were identified and assessed through
transcriptome analysis. The annotation of total unigenes sequences with NR (nonredundant)
database, GO (gene ontology) analysis and COG (Clusters of Orthologous
Groups) analysis were 96.74%, 52.28%, and 29.44% respectively. During HB-1 vs
MB-1, HB-2 vs MB-2 and HB-2 vs MB-3 stages, more numbers of ethylene and auxin
transcripts were up-regulated and among the protein kinases, LRR receptor-like
serine/threonine-protein kinase, putative receptor-like protein kinase, CBL-interacting
protein kinase and serine/threonine-protein kinase related transcripts were upregulated.
Upregulation of LDL2, TPL and WRKY transcripts at bud stages (HB-1 VS
MB-1) and UBP-12, MYB30, AGL-24 and early flowering genes (ELF, EFS, REF6)
at panicle development stages (HB-2 VS MB-2 & MB-3) were identified. More numbers
of transcription factors related to WRKY (12), followed by ERF (6), and MYB (5) were
up-regulated at HB-1 vs MB-1, HB-2 vs MB-2 and HB-2 vs MB-3 stages. The KEGG
pathway analysis indicated that more number of differentially expressed transcripts for
phenylpropanoid biosynthesis, flavonoid biosynthesis, amino acid pathway, oxidative
phosphorylation, plant hormone signal transduction, AMPK, MAPK, and VEGF
signaling pathways were observed at HB-1 vs MB-1, HB-2 vs MB-2 and HB-2 vs MB3
stages. Gene networking analysis of DEG’s inferred that HSP, SHMT, SOD, WRKY
33, MAPK, CCR, and LHY5 were actively involved in defence mechanism of healthy
tissue (HB-1 & HB-2). Out of 65 Fusarium resistance gene sequences studied, 33
protein sequences were stable. The most commonly occurring protein motifs were
motif-3 and motif-1. More number of Fusarium elicitor responsive CRE’s (Box-W1
and EIRE) and biotic stress responsive CRE’s (TC-rich repeats, CGTCA-motif, ATrich
sequence and TGACG-motif) were also observed in Fusarium resistance genes.
These findings will serve as foundation for future functional studies and in genetic
improvement programmes to develop variety resistant to mango malformation.
 
Date 2018-10-09T09:22:46Z
2018-10-09T09:22:46Z
2017
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/5810077630
 
Language en_US
 
Format application/pdf
 
Publisher Division of Fruits and Horticultural Technology ICAR-Indian Agricultural Research Institute New Delhi