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Cloning and characterization of phytochrome genes in chickpea

KrishiKosh

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Title Cloning and characterization of phytochrome genes in chickpea
 
Creator REVATHY CHARAGONDA
 
Contributor P.K. Jain
 
Subject null
 
Description t-9783
Phytochromes are a family of related chromoproteins that regulate
photomorphogenesis in plants. overexpression of the phytochrome A in several
plant species has pleiotropic effects. Phytochromes sense red/far red light and
trigger a cascade of physiological responses in plant. Here, a PHYB homolog
CaPHYB was amplified from the chickpea genome and its expression profiles were
obtained for various parts of the plant. The gene was over expressed in Arabidopsis
thaliana, driven by CaMV35S promoter, to study the physiological functions of the
gene product. As expected, over expression of chickpea functional CaPHYA1 in
transgenic Arabidopsis under the control of the cauliflower mosaic virus (CaMV)
35s promoter reduced hypocotyls growth under white light, caused semi dwarfism,
decreased apical dominance, it enhanced chlororophyll biosynthesis and
anthocyanin accumulation. Higher number of smaller but thicker leaves increased
pigmentation in transgenic Arabidopsis. The beginning of senescence was not
delayed, but deceleration of chlorophyll degradation extended the lifetime of
photosynthetically active plants.Transgenic Arabidopsis plants over expressing
chickpea PHYB were characterized in a green house to explore their potential for
improved photosynthesis. As expected, over expression of functional CaPHYB
caused pleiotropic effects such as semi dwarfism decreased apical dominance, a
higher number of smaller but thicker leaves and increased pigmentation. Both the
higher photosynthetic performance and the longer lifespan of the transgenic plants
allowed greater biomass production. CaPHYE1 expressed at a relatively higher
level in the seedlings, suggesting that it might have an important role in seed
germination and seedling growth.
 
Date 2018-10-09T09:11:43Z
2018-10-09T09:11:43Z
2017
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/5810077628
 
Language en_US
 
Format application/pdf
 
Publisher NATIONAL RESEARCH CENTRE ON PLANT BIOTECHNOLOGY ICAR-INDIAN AGRICULTURAL RESEARCH INSTITUTE NEW DELHI