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PROGRESSIVE STUDIES IN THE MOLECULAR PATHOLOGY OF EXPERIMENTALLY INDUCED HYPOTHYROIDISM AND EVALUATION OF PROTECTIVE ROLES OF SHILAJIT AND WITHANIA SOMNIFERA IN RATS

KrishiKosh

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Title PROGRESSIVE STUDIES IN THE MOLECULAR PATHOLOGY OF EXPERIMENTALLY INDUCED HYPOTHYROIDISM AND EVALUATION OF PROTECTIVE ROLES OF SHILAJIT AND WITHANIA SOMNIFERA IN RATS
 
Creator RAMYA. B
 
Subject organic compounds, diseases, biological phenomena, concentrates, proteins, irrigation, research methods, enzymes, organic acid salts, nucleus
 
Description Endocrine disorders are widespread among Indian inhabitants and thyroid disorders represents an imperative subset of these endocrine disorders. Hypothyroidism is a clinical entity resulting from insufficient production or diminished action of either T3 and/or T4 thyroid hormones and high plasma levels of thyroid stimulating hormone (TSH). Hypothyroidism have relation with several system functions in the body which wind up in multiple organ defects in the affected species. The current study was conducted to appraise ameliorative effect of herbs, Withania somnifera and Shilajit either solely or in combination.
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The study was conducted in 21 days old weaned female Sprague-drawly rats (96) divided into 8 groups with 12 in each group for a period of 3 months. Hypthyroidism was induced by administering methimazole at the rate of 0.02%, ameliorating agents Shilajt and Withania somnifera @100 mg/Kg b. wt. were used. Standard treatment group with levothyroxine (LT4) along with control group and herb standard controls were maintained or compared.
Average body weights were recorded in all the groups at monthly intervals. Blood samples were procured at monthly intervals for estimation of blood glucose and lipid profile. Thyroid profile and oestrogen levels were estimated from sera of blood collected at the end of third month. At the end of 3 months, 6 rats from each group were sacrificed and various organs (heart, kidney, brain, liver, uterus, ovary and thyroid) were collected in suitable preservatives for histopathological and electron microscopic (thyroid) studies. The tissues of heart, kidney and brain were stored at -200C for further estimation of GSH, TBARS and protein carbonyls in homogenates. The activity of glycogen synthase and glucose-6-phosphate dehydrogenase (G6PD) were estimated in the liver homogenate.
Remaining 48 female rats (6 from each group) of the experiment 1 were mated with 24 euthyroid adult (above 3 months) males and all the 48 pregnant rats were administered with drugs used as above.
Progesterone levels in pregnant dams were estimated. Pups from all the groups were screened for survival and mortality rates. Half the number of the pups from each group on zero day of their birth were preserved in 10% neutral buffered formalin for histopathology of wholepup as a unit and for skeletal staining to view the teratogenicity.
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Remaining half of the pups were reared till 21 days to study neuro behavioral parameters such as elevated plus maze and Morris water maze. The rats were then sacrificed and various organs (heart, kidney, brain, uterus, testis and thyroid) were collected in suitable preservatives for histopathology and electron microscopy (brain and thyroid ) studies.
The mean monthly body weights, glutathione in brain, heart and kidney, serum T3, T4 concentrations, glucose-6-phosphate dehydrogenase and glycogen synthase in hypothyroid group and efficiency in neurobehaviour parameters like elevated plus maze and Morris water maze in the pups born to hypothyroid rats were significantly (p
 
Date 2016-12-27T14:50:08Z
2016-12-27T14:50:08Z
2016-09-15
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/93265
 
Relation D;479
 
Format application/pdf
 
Publisher PVNR TVU