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Genetic Characterization of Mahabubnagar Goats based on Microsatellite Markers

KrishiKosh

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Title Genetic Characterization of Mahabubnagar Goats based on Microsatellite Markers
 
Creator RAGHAVENDRA CHOWDAM
 
Contributor Dr. S. SAI REDDY
 
Subject alleles, livestock, locus, genetics, genetic structures, breeds (animals), polymorphism, dna, genotypes, pcr
 
Description The present study was undertaken for assessment of genetic diversity in Mahabubnagar
goats through DNA fingerprinting using a total of 15 microsatellite markers selected from the
list suggested by FAO. The genomic DNA was isolated using standard phenol-chloroform
method which had a mean optical absorbance ratio (260/280nm) of 1.903 while the mean
quantity of DNA was 0.826 μg/μl. All the 15 microsatellites were highly polymorphic.
A total of 132 alleles were observed across the 15 loci amplified. The number of alleles
at a locus varied from 5 (SRCRSP5) to 13 (SRCRSP15) with an average of 8.800. The allele
size varied from 98 for Locus SRCRSP23 to 296 for ILSTS011. The effective number of alleles
varied from 4.429 (SRCRSP5) to 11.250 (SRCRSP15) whereas the overall mean effective
number of alleles was 7.711. The allele frequency varied from 0.016 (231 bp at CSRD247) to
0.304 (172 bp at SRCRSP5).
The overall mean observed heterozygosity, expected heterozygosity and unbiased
expected heterozygosity values were 0.694, 0.862 and 0.876, respectively. The overall mean
PIC was 0.845 indicating that amplified 15 loci were highly informative. The PIC values for
all the loci were above 0.50. The overall mean Shannon’s information index was 2.071 and it
varied from 1.546 (SRCRSP5) to 2.491 (SRCRSP15). The overall mean outcrossing rate was
0.707 and the values among the 15 loci studied varied from 0.273 (MAF065) to 1.047
(OarFCB48). In the present study, all the 15 loci studied were found to be polymorphic in
nature, thus recording 100% polymorphism.
The overall mean inbreeding coefficient was 0.196. Of the 15 loci studied, only two
loci (OarFCB48, SRCRSP3) showed negative inbreeding coefficient (-0.023 and -0.005)
indicating the presence of outbreeding. Thirteen loci showed positive, mild to moderate
inbreeding which varied from 0.049 to 0.571 studied. Out of the 15 amplified loci, nine loci
deviated significantly from Hardy-Weinberg equilibrium which might be due to heterozygote
deficiency and selection followed.
The results of this study provide valuable information about the genetic structure of
Mahabubnagar goat breeds. The results revealed high genetic variation within the breeds.
However, further studies are required to identify association of markers with desirable traits
such as high prolificacy for which this population is known to exploit the potential of the
Mahabubnagar goats.
 
Date 2016-12-31T15:13:50Z
2016-12-31T15:13:50Z
2016-03-05
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/93826
 
Relation D;456
 
Format application/pdf
 
Publisher PVNR TVU