Production, Optimization and Characterization of Thermostable Cellulase from Aspergillus fumigatus AA001 and its Application in Production of Reducing Sugars from Agriculture Waste
KrishiKosh
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Title |
Production, Optimization and Characterization of Thermostable Cellulase from Aspergillus fumigatus AA001 and its Application in Production of Reducing Sugars from Agriculture Waste
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Creator |
Srivastava, Neha
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Contributor |
Ramteke, Prof. (Dr.) Pramod W
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Subject |
sowing, planting, developmental stages, yields, genotypes, groundnuts, harvesting, root nodulation, land resources, germinability
biofuels, Lignocellulosic, waste, biomass, cellulase |
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Description |
A Thesis on “Production, Optimization and Characterization of Thermostable Cellulase from Aspergillus fumigatus AA001 and its Application in Production of Reducing Sugars from Agriculture Waste” submitted in the partial fulfillment of the requirements for the degree of Doctor of Philosophy in Biotechnology” by Neha Srivastava.
The production and utilization of biofuels is gaining attention worldwide because of several advantages such as reducing global warming and global energy crises. Biofuel such as; bioethanol and biohydrogen can be produced by the fermentation process of sugars obtained from cellulosic materials e.g. agro-industrial wastes. Lignocellulosic biomass is the most abundant, renewable and low-cost source available for the production of bioenergy. Lignocellulosic agricultural wastes biomasses are being used as the substrate for the production of second-generation biofuels. Lignocellulosic biomass is a complex system which is composed of cellulose, hemicellulose & lignin, and the conversion efficiency of lignocellulosic biomass into biofuels is high, excluding lignin. Cellulose and hemicellulose which covers ~twothird of the lignocellulosic biomass are regarded as the potential sources of sugars production. For conversion of these cellulosic materials into the fermentable sugars, lignocellulosic enzymes; cellulases are required. Dynamic researches on cellulases have explored their potential for different industrial sectors. Cellulases are the group of enzymes and can be divided into three major groups: exoglucanase, endoglucanase and β-glucosidases. These enzymes are needed for enzymatic hydrolysis of cellulosic biomass for the generation of fermentable sugars. Generally, the enzymatic hydrolysis reactions are carried out at 45oC-50oC which shows slow enzymatic hydrolysis rates, low yields of sugars, and incomplete hydrolysis and is very sensitive to microbial contamination. These limitations could be resolved by using the thermophilic/thermotolerant microorganism for the production of thermophilic/thermostable enzymes. Thermophilic/thermostable cellulase enzymes have the number of commercial applications, as the paper processing industries are always interested in such type of cellulases which can withstand higher temperatures. In addition, one of the most important applications of thermostable cellulase is in the bioconversion of cellulosic biomass into the fermentable sugars for biofuels production at elevated temperature. To accomplish the aim, “Production, Optimization and Characterization of thermostable crude cellulase viii from Aspergillus fumigatus AA001 and its application in the production of reducing sugars from agriculture waste" the present work has been arranged into five chapters. Chapter-I, includes the general introduction on cellulase and its role in biofuels production process, cost analysis along with its industrial importance and various possible ways to reduce the cost of its production. This chapter also provides knowledge about the potential cellulase producing fungal strain, types of cellulase and its properties like thermal stability and pH stability. Moreover, the role of process parameters to improve the cellulase production and lignocellulosic biomass and its importance for lowering the cost of cellulase as well as overall biofuels production process has been discussed. Chapter-II, presents the review of literature providing broad knowledge on celulases. It also provides the information on the market analysis for cellulases and various factors, responsible for the thermal stability of cellulases. These chapters also summarize several studies for the isolation of thermophilic/thermotolerant microorganisms for thermophile/thermostable cellulase production. Additionally, process parameters affecting the thermophilic/thermosable cellulase production and ways to improve the production process have also been discussed. At the end of this chapter, objectives of the present work are also provided. Chapter-III includes the materials and methodology adopted in the entire study. It describes the isolation, screening, and identification of thermophilic/thermotolerant fungal microbes for the production of thermophilic/thermostable cellulase enzyme. The medium composition used for the production of enzyme, the classical and statistical methods of media optimization for cellulase production, partial purification, biochemical and molecular characterization of crude cellulase are also discussed. Further, the enzymatic hydrolysis using the pretreated agriculture biomass at different substrate loading and the optimal condition is explained. Materials and methods emphasizing the production, thermal stability, and hydrolysis efficiency are also provided. Finally, various analytical tools for the identification of selected microorganism, qualitative, and quantitative analysis method are also summarized. Chapter-IV, describes the results and its discussion obtained from various experimental studies, carried out in the entire study. All the findings of the present ix study have been presented in the forms of figures & tables along with a thorough discussion. The obtained results have also been compared with the previous studies and the existing cellulase produced from other thermophilic/thermotolerant fungus. Finally, the work done in the present study has been summarized in Chapter V. The overall conclusion has been drawn on the basis of the experimental results followed by the future scope of thermostable cellulase and its applications. |
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Date |
2016-12-14T14:45:18Z
2016-12-14T14:45:18Z 2016 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/90150
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Language |
en
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Format |
application/pdf
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Publisher |
Sam Higginbottom Institute of Agriculture, Technology & Sciences (SHIATS)
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