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Production, Optimization and Characterization of Thermostable Cellulase from Aspergillus fumigatus AA001 and its Application in Production of Reducing Sugars from Agriculture Waste

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Title Production, Optimization and Characterization of Thermostable Cellulase from Aspergillus fumigatus AA001 and its Application in Production of Reducing Sugars from Agriculture Waste
 
Creator Srivastava, Neha
 
Contributor Ramteke, Prof. (Dr.) Pramod W
 
Subject sowing, planting, developmental stages, yields, genotypes, groundnuts, harvesting, root nodulation, land resources, germinability
biofuels, Lignocellulosic, waste, biomass, cellulase
 
Description A Thesis on “Production, Optimization and Characterization of Thermostable Cellulase from Aspergillus fumigatus AA001 and its Application in Production of Reducing Sugars from Agriculture Waste” submitted in the partial fulfillment of the requirements for the degree of Doctor of Philosophy in Biotechnology” by Neha Srivastava.
The production and utilization of biofuels is gaining attention worldwide
because of several advantages such as reducing global warming and global energy
crises. Biofuel such as; bioethanol and biohydrogen can be produced by the
fermentation process of sugars obtained from cellulosic materials e.g. agro-industrial
wastes. Lignocellulosic biomass is the most abundant, renewable and low-cost source
available for the production of bioenergy. Lignocellulosic agricultural wastes
biomasses are being used as the substrate for the production of second-generation
biofuels. Lignocellulosic biomass is a complex system which is composed of cellulose,
hemicellulose & lignin, and the conversion efficiency of lignocellulosic biomass into
biofuels is high, excluding lignin. Cellulose and hemicellulose which covers ~twothird
of the lignocellulosic biomass are regarded as the potential sources of sugars
production. For conversion of these cellulosic materials into the fermentable sugars,
lignocellulosic enzymes; cellulases are required.
Dynamic researches on cellulases have explored their potential for different
industrial sectors. Cellulases are the group of enzymes and can be divided into three
major groups: exoglucanase, endoglucanase and β-glucosidases. These enzymes are
needed for enzymatic hydrolysis of cellulosic biomass for the generation of
fermentable sugars. Generally, the enzymatic hydrolysis reactions are carried out at
45oC-50oC which shows slow enzymatic hydrolysis rates, low yields of sugars, and
incomplete hydrolysis and is very sensitive to microbial contamination. These
limitations could be resolved by using the thermophilic/thermotolerant microorganism
for the production of thermophilic/thermostable enzymes. Thermophilic/thermostable
cellulase enzymes have the number of commercial applications, as the paper
processing industries are always interested in such type of cellulases which can
withstand higher temperatures. In addition, one of the most important applications of
thermostable cellulase is in the bioconversion of cellulosic biomass into the
fermentable sugars for biofuels production at elevated temperature. To accomplish the
aim, “Production, Optimization and Characterization of thermostable crude cellulase
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from Aspergillus fumigatus AA001 and its application in the production of reducing
sugars from agriculture waste" the present work has been arranged into five chapters.
Chapter-I, includes the general introduction on cellulase and its role in
biofuels production process, cost analysis along with its industrial importance and
various possible ways to reduce the cost of its production. This chapter also provides
knowledge about the potential cellulase producing fungal strain, types of cellulase and
its properties like thermal stability and pH stability. Moreover, the role of process
parameters to improve the cellulase production and lignocellulosic biomass and its
importance for lowering the cost of cellulase as well as overall biofuels production
process has been discussed.
Chapter-II, presents the review of literature providing broad knowledge on
celulases. It also provides the information on the market analysis for cellulases and
various factors, responsible for the thermal stability of cellulases. These chapters also
summarize several studies for the isolation of thermophilic/thermotolerant
microorganisms for thermophile/thermostable cellulase production. Additionally,
process parameters affecting the thermophilic/thermosable cellulase production and
ways to improve the production process have also been discussed. At the end of this
chapter, objectives of the present work are also provided.
Chapter-III includes the materials and methodology adopted in the entire study.
It describes the isolation, screening, and identification of thermophilic/thermotolerant
fungal microbes for the production of thermophilic/thermostable cellulase enzyme.
The medium composition used for the production of enzyme, the classical and
statistical methods of media optimization for cellulase production, partial purification,
biochemical and molecular characterization of crude cellulase are also discussed.
Further, the enzymatic hydrolysis using the pretreated agriculture biomass at different
substrate loading and the optimal condition is explained. Materials and methods
emphasizing the production, thermal stability, and hydrolysis efficiency are also
provided. Finally, various analytical tools for the identification of selected
microorganism, qualitative, and quantitative analysis method are also summarized.
Chapter-IV, describes the results and its discussion obtained from various
experimental studies, carried out in the entire study. All the findings of the present
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study have been presented in the forms of figures & tables along with a thorough
discussion. The obtained results have also been compared with the previous studies
and the existing cellulase produced from other thermophilic/thermotolerant fungus.
Finally, the work done in the present study has been summarized in Chapter V.
The overall conclusion has been drawn on the basis of the experimental results
followed by the future scope of thermostable cellulase and its applications.
 
Date 2016-12-14T14:45:18Z
2016-12-14T14:45:18Z
2016
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/90150
 
Language en
 
Format application/pdf
 
Publisher Sam Higginbottom Institute of Agriculture, Technology & Sciences (SHIATS)