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GROSS, HISTOLOGICAL AND HISTOCHEMICAL STUDIES ON STOMACH OF THE PIG (Sus scrofa domesticus)

KrishiKosh

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Title GROSS, HISTOLOGICAL AND HISTOCHEMICAL STUDIES ON STOMACH OF THE PIG (Sus scrofa domesticus)
 
Creator K.SUJANA
 
Contributor Dr. N.RAJENDRANATH
 
Subject agreements, contract farming, crops, costs, productivity, manpower, marketing, area, markets, land resources
 
Description Present work “Gross, histological and histochemical studies on stomach of the Pig (Sus scrofa domesticus)” was done in Department of Veterinary Anatomy, College of Veterinary Science, Hyderabad in six adult pigs irrespective of their sex. Tissue samples were collected at slaughter from local abattoirs around Hyderabad. Gross anatomical features and results were recorded and photographed. Histological features were observed in tissues fixed in 10% NBF and Bouin‟s fluid for routine paraffin method. Paraffin sections were stained with H & E, Masson‟s trichrome, Van Geison‟s, Wilder‟s reticular and Schultz techniques. For histochemical enzyme studies fresh cryostat sections were stained with Oil-red-O for lipids and PAS, PAS-AB, ALP, ACP and SDH methods.
Morphological features of stomach of the pig revealed that it was „C‟ shaped hollow organ with its long axis in transverse direction and its greater curvature extending to the floor of abdomen midway between xiphoid cartilage and umbilicus. Wide infundibular esophageal opening at the cardia continued into inner mucosal surface demarcated into four multi colored regions small whitish non-glandular portion, followed by light grey cardiac region, reddish
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brown fundic zone and lastly pinkish white pyloric region which had a prominent „torus pyloricus‟ at its sphincter. Mean length of greater and lesser curvature was 53 ± 7.25 and 11.88 ± 2.63 cm respectively whereas average weight and volume of stomach in adult pigs was 0.56 ± 0.06 kg and 2.7 ± 1.05 liters respectively.
Histological studies of pig stomach showed four layers viz., tunica mucosa, sub-mucosa, muscularis and serosa. Stratified squamous epithelium above lamina propria devoid of glands changed abruptly to simple columnar epithelium at esophageo-cardiac junction which was abrupt. Cardiac glands were distributed throughout lamina propria. Fundic region showed rugae between which gastric pits opened to fundic glands populated with parietal and chief cells. Relatively thick submucosa and tunica muscularis consisted extremely thick inner circular and thin outer longitudinal layer of smooth muscle. Pyloric mucosa was thicker than cardiac and fundic regions. Closely placed pyloric glands in lamina propria had numerous mast cells. Lamina muscularis had smooth muscle with lymphatic infiltration upto sub mucosa. Outer serosa was thick fibroelastic connective tissue lined by mesothelium.
Histochemical features of pig stomach showed moderate to strong PAS and PAS-AB reaction indicating presence of acid mucopolysaccharides in three regions. Moderate ACP and AKP reaction in epithelium and lamina muscularis was noted in all three gastric regions. Moderate to intense SDH activity occured in cardiac and fundic portions. Diffuse Oil red O reaction was seen in peripheral zones of cardiac and pyloric glands.
 
Date 2017-01-10T15:33:39Z
2017-01-10T15:33:39Z
2017-01-09
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/95374
 
Relation D;516
 
Format application/pdf
 
Publisher PVNR TVU