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GENETIC DIVERSITY STUDIES IN BRINJAL (Solanum spp.) USING MORPHOLOGICAL, BIO-CHEMICAL AND MOLECULAR MARKERS

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Title GENETIC DIVERSITY STUDIES IN BRINJAL (Solanum spp.) USING MORPHOLOGICAL, BIO-CHEMICAL AND MOLECULAR MARKERS
 
Creator Paithankar, Dinesh H
 
Contributor Kothe, Shri R. K.
Bharad, Dr. S. G.
Deshmukh, Dr. D. T.
 
Subject null
 
Description The present investigation enti tled ""Genetic diversity studies in
brinjal (Solanum spp.) using morphological, bio-chemical and molecular
markers"" was undertaken with the objectives to estimate the diversity
among 26 genotypes of brinjal (Solamun spp.) using morphological. biochemical
and molecular markers and find the suita bil ity of different marker
systems to study the genetic diversity in brinjal for their uti liza tion in
hybridization programme.
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The analysis of variance revealed significant differences among
the genotypes for all the characters under study.
Canonical analysis indicated about 92.44 per cent of total
variation was accounted by the four canonical roots. Fruits per cluster
(vector 1), plant height (vector II), days to 1"" harvest (vector Il l) and fruit
length (vector IV) were important sources of variation on respective vectors
and also confirmed by cluster mean for different characters.
Seed protein profi le was studied by SDS-PAGE and esterase as
well as peroxidase profile using Native PAGE technique.
RAPD technique was used to assess genetic diversity by means
of molecular markers. A total 173 polymorphic amplified products were
obtained from 20 decamer primers which discriminated all the accession.
All 26 genotypes were grouped on the basis morphological
markers (three clusters), protein markers (seven clusters), esterase
markers (four clusters), peroxidase markers (three clusters) and RAPD
markers (nine clusters).
Clustering done by morphological marker were not in agreement
with biochemical and molecular markers. Diagnostic RAPD's were
identified which discriminate among the Solanum species. Two brinjal
accessions misclassified based on morphological traits were reassigned
individual cluster using RAPD's. Similarly, protein markers next to the
molecular marker could class ify Solanum species to the different cluster
precisely.
Different cross combinations were suggested on the basis of
protein markers (81) esterase markers (146) and RAPD markers (133).
However, the common cross combinations in protein , esterase and RAPD
markers reduced the number of cross combinations to twenty five to expect
relatively good amount of heterosis in F, and to get high frequency of
transgressive segregants in subsequent generation which would be further
utilized in breeding programme.
Using RAPD marker, a total 15 unique bands were observed in a
set of brinjal genotypes which could be uti lized for the identification of
genotypes.
RAPD primers OPA-04, OPA-05, OPA-16, OPB 10, OPB 12,
OPH 02 , OPH 04 and OPH 05 shown highest polymorphism in a set of
brinjal genotypes, hence these primers were most useful for the
discrimination of brinjal genotypes.
The present study indicates usefulness of assessing genetic
diversity at DNA level using RAPD markers and its potential to determine
the similarity between genotypes more precisely as compared to
biochemical and morphological markers.
 
Date 2018-10-17T10:00:20Z
2018-10-17T10:00:20Z
2011
 
Type Thesis
 
Identifier PAITHANKAR DINESH HARIDAS (2011), GENETIC DIVERSITY STUDIES IN BRINJAL (Solanum spp.) USING MORPHOLOGICAL, BIO-CHEMICAL AND MOLECULAR MARKERS, DEPARTMENT OF HORTICULTURE POST GRADUATE INSTITUTE, AKOLA, DR. PANJABRAO DESHMUKH KRISHI VIDYAPEETH, KRISHINAGAR PO, AKOLA, 2011 , 233p
http://krishikosh.egranth.ac.in/handle/1/5810081703
 
Language en
 
Format application/pdf
 
Publisher Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra.