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Proteomic and transcriptomic approaches to identify resistance and susceptibility related proteins in contrasting rice genotypes infected with fungal pathogen Rhizoctonia solani

OAR@ICRISAT

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Relation http://oar.icrisat.org/10905/
http://dx.doi.org/10.1016/j.plaphy.2018.07.012
10.1016/j.plaphy.2018.07.012
 
Title Proteomic and transcriptomic approaches to identify resistance and susceptibility related proteins in contrasting rice genotypes infected with fungal pathogen Rhizoctonia solani
 
Creator Prathi, N B
Palit, P
Madhu, P
Ramesh, M
Laha, G S
Balachandran, S M
Madhav, M S
Sundaram, R M
Mangrauthia, S K
 
Subject Rice
Genetics and Genomics
 
Description The devastating sheath blight disease caused by Rhizoctonia solani Kuhn (teleomorph: Thanatephorus cucumeris) causes major yield loss in most rice growing regions of the world. In this study, two moderately tolerant and four susceptible genotypes of rice were selected for R. solani induced proteome analysis using two-dimensional polyacrylamide gel electrophoresis. Forty five differentially expressed proteins (DEPs) were identified and analyzed by Mass Spectrometry. Based on their functions, these proteins were classified into different groups, viz., photosynthesis, resistance and pathogenesis, stress, cell wall metabolism and cytoskeleton development associated proteins, and hypothetical or uncharacterized proteins. Expression of 14 genes encoding DEPs was analyzed by quantitative PCR which showed consistency in transcripts and genes expression pattern. Furthermore, the expression of 16 other genes involved in diverse biological functions was analyzed. Up-regulation of these genes in the tolerant genotype Pankaj during sheath blight disease suggested efficient genetic regulation of this cultivar under stress. Also, expression analysis of conserved microRNAs (miRNAs) and their target genes revealed important role of miRNAs in post-transcriptional gene regulation during development of rice sheath blight disease. Genome-wide discovery of miRNAs and further characterization of DEPs and genes will help in better understanding of the molecular events during sheath blight disease development in rice.
 
Publisher Elsevier
 
Date 2018-09
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/10905/1/Plant%20Physiology%20and%20Biochemistry-%20Paramita.pdf
Prathi, N B and Palit, P and Madhu, P and Ramesh, M and Laha, G S and Balachandran, S M and Madhav, M S and Sundaram, R M and Mangrauthia, S K (2018) Proteomic and transcriptomic approaches to identify resistance and susceptibility related proteins in contrasting rice genotypes infected with fungal pathogen Rhizoctonia solani. Plant Physiology and Biochemistry (TSI), 130. pp. 258-266. ISSN 09819428