Record Details

Environmental DNA (eDNA) metabarcoding approach in fisheries research in India

CMFRI Repository

View Archive Info
 
 
Field Value
 
Relation http://eprints.cmfri.org.in/13065/
 
Title Environmental DNA (eDNA) metabarcoding approach in fisheries research in India
 
Creator Jayasankar, P
Pradeep, M A
Mini, K G
Arun Kumar, T V
 
Subject Fish Biotechnology
 
Description Environmental DNA (eDNA) is defined as the
genetic material obtained from a water sample
containing no distinguishing signs of source macroorganisms.
The method utilizes DNA which is
continuously excreted by organisms into the
surrounding environment through mucus, gamates,
faeces, blood and other cells, and captures, analyses
and obtains the nucleotide sequence of this DNA
based on an environmental sample. eDNA analysis
has emerged as a potentially powerful tool to access
aquatic community structures. Analysis of eDNA can
give us information on the organisms, their
abundance and biomass through two approaches –
eDNA barcoding and eDNA metabarcoding. In the
former, specific species are targeted in samples
using standard or quantitative PCR, and using
traditional Sanger sequencing method.
 
Publisher Central Marine Fisheries Research Institute
 
Date 2017
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/13065/1/MFIS-234_11.pdf
Jayasankar, P and Pradeep, M A and Mini, K G and Arun Kumar, T V (2017) Environmental DNA (eDNA) metabarcoding approach in fisheries research in India. Marine Fisheries Information Service; Technical and Extension Series (234). pp. 29-31. ISSN 0254-380 X