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Selective identification of specific aptamers for the detection of non-typhoidal salmonellosis in an apta-impedimetric sensing format

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Selective identification of specific aptamers for the detection of non-typhoidal salmonellosis in an apta-impedimetric sensing format
 
Creator Pathaniya, Preeti
Sharma, Anurima
Kumar, Binod
Rishi, Praveen
Suri, C Raman
 
Subject QR Microbiology
 
Description The authors report on an aptamer-based method for the detection of S. Typhimurium. The aptamers were identified by using a modified cell-based SELEX method (cell-SELEX) by adopting an alternating negative-positive selection strategy. Each cross-reactive population was used separately, with a focus on generating strain-specific aptamers in order to enhance selectivity. The aptamers were characterized and chemically modified with thiol groups at the 5′ end which enables covalent binding to gold nanoparticles on a screen printed carbon electrode (GNP-SPCE). The aptamer-modified GNP-SPCE was subsequently applied to impedimetric sensing of S. Typhimurium in water and food samples. The response is linear in the 10 to 105 CFU⋅mL−1 concentration range, and the limit of detection is ~10 CFU⋅mL−1. The assay can distinguish S. Typhimurium from S. Typhi and has been validated in water and spiked egg samples.
 
Publisher Springer
 
Date 2017-05
 
Type Article
PeerReviewed
 
Relation https://link.springer.com/article/10.1007/s00604-017-2098-2
http://crdd.osdd.net/open/2126/
 
Identifier Pathaniya, Preeti and Sharma, Anurima and Kumar, Binod and Rishi, Praveen and Suri, C Raman (2017) Selective identification of specific aptamers for the detection of non-typhoidal salmonellosis in an apta-impedimetric sensing format. Mcrichimica Acta, 184 (5). pp. 1499-1508.