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CLONING AND CHARACTERIZATION OF IRON REGULATED TRANSPORTER GENE (IRT) – ASSOCIATED WITH GRAIN Fe CONTENT FROM LITTLE MILLET (Panicum sumatrense)
KrishiKosh
View Archive Info| Field | Value | |
| Title |
CLONING AND CHARACTERIZATION OF IRON REGULATED TRANSPORTER GENE (IRT) – ASSOCIATED WITH GRAIN Fe CONTENT FROM LITTLE MILLET (Panicum sumatrense)
M.Sc. Biotechnology |
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| Creator |
Alagarasan, G.
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| Contributor |
Chandel, G.
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| Subject |
Cloning, IRT, Genomics
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| Description |
Genes in the ZIP family encode transcripts to procure and transport bivalent metal micronutrient(s), particularly Fe and or Zn. It constitutes the variety of functions involved in the developmental and physiological processes in many plant species, if not all, Poaceae plant species as well as the model species Arabidopsis. We present herein the report of genome wide investigation of orthologous ZIP genes in Setaria italica and identified 7 seven potential genes to possibly increase the bio-availability of zinc and iron content in grains. Out of seven single copy ortholog members of Rice and Arabidopsis, six of them were functionally active. Among 36 ZIP members, 25 genes have traces of signal peptide based sub-cellular localization, as compared to those of plant species studied hitherto, yet vasculature of ions remains unclear. In silico analysis of gene and protein modeling suggested that these two different gene structure and protein natures were preeminent in shaping the functional diversity of the ZIP gene family in S. italica. Phylogenetic analysis uncovered that speciation and duplication events caused gene family development and ensuring the non-transitivity nature. Finally, this study provides new insights into the signal peptide based/ independent iron and zinc homeostasis in the plant system and allowed identification of ZIP genes that may involved in the zinc and iron absorption from the soil and thus transporting it to the cereal grain underlying high micronutrient accumulation. Engineering plant system to increase metal ion vasculature efficiency and reduce iron avoidance response is very important for further bio-fortification of crop plants. Identification of potential genes controlling iron homeostasis in micronutrient(s) rich crop is needed to provide insight into molecular mechanism of high iron accumulation and achieving ideal plant system with high grain nutrients. The transcriptome assay of single copy ortholog ZIP gene family, cloning and sequencing of the IRT gene in different genotypes was done in Panicum sumatrense having high zinc/ iron content. Comparative genomic analysis was performed with Rice, Arabidopsis and Foxtail millet. Cloned gene was named as PsIRT2 (Panicum sumatrense Iron Regulated Transporter gene 2). The iron vasculature is possibly controlled by a gene PsIRT2, has 2 exons encoding a protein with 226-242 amino acids and its domain are the same as those of the rice OsIRT2 protein. PsIRT2 was found to locate in the mitochondria while OsIRT2 in secretary pathway. Though PsIRT2 is lack of signal peptide, the grain Fe and Zn content is very high in little millet as compared to rice grains. The number of Trans membrane domain in PsIRT2 is also lesser than OsIRT2 playing significant role in metal influx. We hypothesize that PsIRT2 is a signal peptide independent ion transporter. Further validation of particular ZIP gene family expression was also carried out in three genotypes, indicating many ZIP genes had been extensively utilized in molecular breeding or crop bio-fortification process. |
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| Date |
2016-08-12T17:23:36Z
2016-08-12T17:23:36Z 2016 |
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| Type |
Thesis
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| Identifier |
82p.
http://krishikosh.egranth.ac.in/handle/1/72442 |
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| Language |
en
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| Format |
application/pdf
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| Publisher |
Indira Gandhi Krishi Vishwavidyalaya, Raipur
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