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ESTRUS SYNCHRONIZATION IN POSTPARTUM ANESTRUS MEHSANA BUFFALOES USING DIFFERENT HORMONE PROTOCOLS AND EARLY PREGNANCY DIAGNOSIS BY TRANSRECTAL ULTRASONOGRAPHY

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Title ESTRUS SYNCHRONIZATION IN POSTPARTUM ANESTRUS MEHSANA BUFFALOES USING DIFFERENT HORMONE PROTOCOLS AND EARLY PREGNANCY DIAGNOSIS BY TRANSRECTAL ULTRASONOGRAPHY
 
Creator NAIKOO, MEHRAJUDDIN
 
Contributor PATEL, D. M.
 
Subject Veterinary Obstetrics and Gynaecology
A Study
 
Description The present study was carried out on 24 postpartum anestrus Mehsana buffaloes during breeding season (November 2008 to March 2009) at Geeta Dairy Farm, Jambuva Village near Jambuva crossing, Vadodara, Gujarat. The animals were divided into four groups (six in each group), keeping six animals as control. The study included administration of three hormone protocols: i) Ovsynch ii) Ovsynch and CIDR and iii) CIDR alone. The effect of these hormone protocols was studied using different parameters and B-mode transrectal ultrasonography and the findings were evaluated by comparing them with plasma progesterone levels at different time intervals after treatment. The Ovsynch protocol was initiated in Mehsana buffaloes with postpartum anestrus period of more than four months, considering the day of first GnRH injection as day 0. All the animals of this group showed synchronized estrus and ovulation. Estrus was observed between 10-20 hrs after the last GnRH injection with ovulation 9 to 15 hrs after the end of estrus in all the animals. The animals were bred by fixed-time AI (FTAI) twice (day 11) and ultrasonography on day 26 post-insemination revealed first service and second service conception rates of 50% each, giving overall conception rate of 66.66% (four out of six animals). Ultrasonography on day 40 and rectal palpation on day 60 post-insemination revealed both pooled conception rate and pregnancy rate of 50% (three out of six animals), suggestive of early embryonic death (EED) in one animal. Combination of Ovsynch-CIDR protocol was initiated in postpartum anestrus animals, considering the first day of treatment as day 0. Synchronization of estrus and ovulation occurred in all the six animals, with ovulation within 10 to 14 hrs after the end of estrus in all the animals. The animals were bred by FTAI and ultrasonography on day 26 post-insemination revealed first service conception rate, second service conception rate and overall conception rate of 66.66% (four out of six animals), 50.00% (one out of two animals), and overall conception rate of 83.33% (five out of six animals), respectively. Both, ultrasound scanning and rectal palpation on day 40 and 60 post-insemination revealed pooled pregnancy rate of 83.33%. The use of CIDR protocol in postpartum anestrus animals caused induction of estrus in all the six animals with ovulation in five out of six animals (83.33%). These animals were bred by FTAI and ultrasonographic scanning on day 26 and 40 postinsemination revealed first service and second service conception rates of 50%) each and pooled conception rate of 66.66% (four out of six animals). The rectal palpation on day 60 post-insemination indicated 50.00% pregnancy rate, suggestive of EED or EFD in one animal. In the control group, all the six animals were anestrus as observed by rectal examination and plasma P4 assay. The plasma P4 levels in all the six animals remained < 1.20ng/ml throughout the research period. Upon ultrasound scanning, the embryo proper was observed on day 26 postinsemination. However, the embryo was observed in nine out of 13 buffaloes. The amniotic and allantoic vesicles were observed as non-echogenic cavities closely surrounding the embryo proper on day 40 post-insemination. These cavities were surrounded by large anechoic area i.e. embryonic fluid. The heartbeats were observed in 10 out of 13 animals on day 26 and in all 12 animals on day 40 post-insemination. The mean heart rate was found to be 174.60 ± 0.81 beats per minute on day 26 postinsemination, which later on decreased to 165.28 ± 1.4 beats per minute on day 40 post-insemination. The sensitivity of ultrasound scanning was cent percent on days 26 and 40 post-insemination. The specificity of ultrasound scanning was 80.00 per cent and 100 per cent on days 26 and 40 post-insemination, respectively. The positive predictive value (PPV) of the ultrasound scanning was 92.85 per cent and 100 per cent on days 26 and 40 post-insemination, respectively. The negative predictive value (NPV) of the ultrasound scanning was cent per cent on both the days i.e. days 26 and 40 post-insemination. The overall accuracy/diagnostic accuracy in the present study was recorded as 94.44 per cent and 100 percent on days 26 and 40, respectively. All the 24 buffaloes included in this study were anestrus, having plasma progesterone levels ranged from 0.21-1.18ng/ml. After initiation of treatment, plasma progesterone levels increased in all animals among the treatment groups upto 9-10 days and then decreased drastically around the day of estrus. Plasma progesterone concentrations in estrus buffaloes ranged from 0.12 - 1.54ng/ml with an average of 0.46±0.20ng/ml on the day of Al. Plasma progesterone concentrations ranged from 3.00 - 6.02ng/ml with an average of 4.42±0.52ng/ml in pregnant animals on day 20 post-insemination. In non-pregnant animals plasma progesterone concentrations remained to be 0.97 - 1.91ng/ml with an average of 1.22±0.06ng/ml on day 20 postinsemination. These results suggest that estrus synchronization in postpartum anestrus Mehsana buffaloes using different hormone protocols viz. Ovsynch, Ovsynch and CIDR and CIDR alone are extremely useful in initiating cyclicity in anestrus/acyclic buffaloes and may serve as an excellent tool for synchronization of estrus, ovulation and conception in postpartum Mehsana buffaloes. Ultrasonography facilitates diagnosis of all non-pregnant animals at an early stage (day 26 post-insemination) and is really advantageous than pregnancy diagnosis by rectal palpation method, with 100 per cent reliability. Thus, ultrasound scanning appeared to be of immense value in detection of early pregnancy, early nonpregnancy and early embryonic mortality.
 
Date 2018-06-11T04:07:09Z
2018-06-11T04:07:09Z
2009
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/5810050632
 
Language en
 
Format application/pdf
 
Publisher AAU, Anand