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Biochemical and molecular basis of seed priming in soybean seed

KrishiKosh

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Title Biochemical and molecular basis of seed priming in soybean seed
 
Creator Pradeepkumar G. B.
 
Contributor N. K. Biradarpatil
 
Subject null
 
Description A laboratory experiments was conducted to study the ‘biochemical and molecular
basis of seed priming in soybean seed’ and to assess the storability of primed seeds of
soybean at Seed Quality and Research Laboratory, NSP. (Crops), UAS, Dharwad. The
experiment was compromised of fresh and old seed lots of soybean Cv. JS– 335 having initial
germination of 90 per cent (High vigour) and 70 per cent (Low vigour) as factor-I and the
different priming treatments such as hydration for two hours (Hydro priming), priming with
GA3 (50 ppm), PEG-6000 (200 g/1000 ml), KH2PO4 (0.5%) as factor-II. Experiments were
laid out with two factorial CRD. The positive effect of priming in improving the seed quality
parameter was better witnessed in low vigour seed lots compared with high vigour seeds.
Though the high vigour seed lots recorded higher germination (95.1%), vigour index (3028),
dry weight of seedlings (110.77 mg) and lower EC (0.677 dS m-1) after priming as compared
to low vigour seed lots which recorded (75.3%), (2067), (81.63 mg) and (1.492) respectively.
The rate of increase in germination percentage was higher in low vigour seed lot (8.82%) as
compared to high vigour seed lot (3.03%). Among the primed seeds, the seeds treated with 50
ppm GA3 recorded higher seed quality parameters viz., germination per cent (88.5), root
length (20.42 cm), shoot length (12.73 cm), seedling vigour index (2947) and seedling dry
weight (101.71 mg). The high vigour seed lot recorded more protein (38.28%) and sugar
content (27.64) as compared to low vigour seed lot which recorded (37.36%) and (27.05%)
respectively. Seeds primed with GA3 after 48 h recorded significantly higher protein
(39.03%) and sugar content (30.72%) as compared to unprimed seeds (36.74%) and (24.54%)
respectively. The GA3 recorded higher amylase (3.03), invertase (2.87) and DNA content
(285.08μg/g) after 48 h of priming as compared to control (2.08), (2.03) and (228.05 μg/g)
respectively.
 
Date 2017-03-06T09:51:59Z
2017-03-06T09:51:59Z
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/5810003979
 
Language en
 
Format application/pdf
 
Publisher UASD