ICAR Research Data Repository for Knowledge Management
TRANSFER OF B-GLUCURONIDASE (Gus) GENEIN SUNFLOWER (HelianthusannuusL.)USING PARTICLE INFLOW APPARATUS
KrishiKosh
View Archive Info| Field | Value | |
| Title |
TRANSFER OF B-GLUCURONIDASE (Gus) GENEIN SUNFLOWER (HelianthusannuusL.)USING PARTICLE INFLOW APPARATUS
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| Creator |
CHARITHA REDDY, G.
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| Contributor |
KULDEEPSINGHDANGI
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| Subject |
null
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| Description |
Inthepresentinvestigation,tissueculturestudieswerecarriedoutto determinetheeffectofdifferenthormonalconcentrationsandcombinations oncallus inductionandgrowthandtodeterminetheresponseofexplants (root,hypocotyl,cotyledonaryleafandmatureembryo)forcallusinduction andgrowth in fivesunflowergenotypesviz., KBSH-l,PCSH—245, 234—A, 245—A and 6D-1. Studies were also conducted to determine the transformationfrequency(intheformoftransientGusexpressionpercent) indirectexplantsandtheirrespectivecalliofthefivesunflowergenotypes usingparticleinflowapparatus. Alltheexplantsofthefivegenotypesrespondedinallthehormonal combinations. However, the callus induction frequency (%) varied from explant to explant and genotype to genotype. Among the four explants, cotyledonary leafwas more responsive for callus induction and growth. Hybrid,KBSH—l followedbyhybrid,PCSH-245wasfoundtobesuperior overothergenotypesintermsofcallusinductionfrequency.Thedifferences observed in callus induction among different explants and the five genotypes may be attributed to the differences in endogenous hormonal concentrations among different explants and differences in genetic constitutionofgenotypes.Amongthe fivehormonaltreatments,MS+0.5 mgl‘1NAA+2.0mg1‘1 BAandMS+ 1.0mgl‘1NAA+0.5mg1'1 BA were found to be better for callus induction. Transformationfrequencyofdirectexplantsandtheirrespectivecalli ofthe fivesunflowergenotypeswasassessedusingreporter(Gus)geneby particle inflow apparatus. Transformation frequency was assessed in the form oftransient Gus expression percentage. Among the four explants, mature embryowas found to givehighesttransient Gusexpression (both direct explantandcallus derived fromit). Direct explants showedhigher transient Gus expression percentage than the callus derived from the explants.Thestudysuggestsbettertransformabilityofdirectexplantsofthe sunflower genotypes using particlebombardment as themethod ofgene transfer. |
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| Date |
2018-06-06T06:29:00Z
2018-06-06T06:29:00Z 2002 |
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| Type |
Thesis
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| Identifier |
D6592
http://krishikosh.egranth.ac.in/handle/1/5810049369 |
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| Language |
en
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| Format |
application/pdf
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| Publisher |
Professor Jayashankar Telangana State Agricultural University
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