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INFLUENCE OF LONG TERM STORAGE OF SEEDS ON GENETIC PURITY OF REDGRAM VARIETY TTB7: A MOLECULAR EVIDENCE

KrishiKosh

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Title INFLUENCE OF LONG TERM STORAGE OF SEEDS ON GENETIC PURITY OF REDGRAM VARIETY TTB7: A MOLECULAR EVIDENCE
 
Creator HUMERA, JABEEN
 
Contributor Ravikumar, R. L.
 
Subject null
 
Description Regeneration of gene bank accessions leads to loss of viability in storage and is
associated with genetic change. The present investigation was aimed to determine the
genetic uniformity of the long term stored seeds of redgram variety TTB7. Twentyfive
long term stored seeds of redgram variety TTB7 (Accession no: IC-296126) was obtained
from NBPGR, New Delhi. Only seventeen out of twenty five long term stored seeds
could germinate and establish. The plants recorded variations particularly in major traits
like plant growth habit, plant height, leaf shape and flower colour among DUS characters.
On the other hand, the fresh nucleus seeds produced only uniform plants. Ten RAPD and
fifteen SSR primers were used to check the uniformity of the nucleus and fresh seeds at
molecular level. Out of ten RAPD primers used, two primers (OPK6 and OPK12)
produced different banding pattern in the plants 1, 2, 6, 7 and 13 of the long term stored
seeds. However, SSR markers produced uniform banding pattern in all the seventeen
plants. The fresh nucleus seeds produced only identical bands for both RAPD and SSR
markers suggesting genetic uniformity in fresh seeds. The progeny plants were tested for
the presence of additional band observed in parental plants using OPK6 and OPK12
primers. The additional band observed in the first generation plant was not observed in
the progeny plants, suggesting that the additional band observed in parental plant could
be erroneous than real. Further studies with bigger sample size may be used to confirm
the results.
 
Date 2018-04-09T04:13:30Z
2018-04-09T04:13:30Z
2017-05-10
 
Type Thesis
 
Identifier Th-11620
http://krishikosh.egranth.ac.in/handle/1/5810043040
 
Language en
 
Format application/pdf
 
Publisher UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU