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IDENTIFICATION OF MOLECULAR MARKERS LINKED TO BACTERIAL WILT IN BRINJAL

KrishiKosh

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Title IDENTIFICATION OF MOLECULAR MARKERS LINKED TO BACTERIAL WILT IN BRINJAL
 
Creator SUBHADA, PATTANAYAK
 
Contributor ASWATH, C
 
Subject ---
 
Description Eggplant is an important vegetable in India and is grown
throughout the tropical, sub tropical and warm temperate regions of the
world. Bacterial wilt caused by Ralstonia solanacearum is a severe soil
borne vascular disease commonly occurring in the warm, humid tropics.
An investigation was conducted to identify molecular markers linked to
bacterial wilt resistance in brinjal in the F2 mapping population
developed by cross between a bacterial wilt resistant variety IIHR-7 with
susceptible variety IIHR-108. 255 F2 plants were phenotyped by artificial
root infection with Ralstonia solanacearum L. (3:1) ratio of resistancesusceptibility
was observed in F2 and the genetic ratio indicated the
resistance trait controlled by a single dominant gene.
39 EST-SSRs, 119 genomic SSRs and 100 RAPDs were used to
genotype parents, F2 population though bulk segregant analysis (BSA).
29 genomic SSRs and 17 RAPDs had shown polymorphism in parental
lines and were further used to screen the mapping population. No
polymorphism was observed in the F2 population. Thus, no marker
linked to bacterial wilt resistance in brinjal was identified in the present
F2 population under study.
Further, linkage analyses are to be performed to identify the
genomic SSRs and RAPDs using more primers that are linked to the
resistance trait in a larger population and integrated into a molecular
marker assisted breeding programme of eggplant to breed bacterial wilt
resistant varieties.
 
Date 2016-12-19T10:52:25Z
2016-12-19T10:52:25Z
2011-08-09
 
Type Thesis
 
Identifier Th-10012
http://krishikosh.egranth.ac.in/handle/1/90914
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences GKVK, Bangalore