IDENTIFICATION OF MOLECULAR MARKERS LINKED TO BACTERIAL WILT IN BRINJAL
KrishiKosh
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Title |
IDENTIFICATION OF MOLECULAR MARKERS LINKED TO BACTERIAL WILT IN BRINJAL
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Creator |
SUBHADA, PATTANAYAK
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Contributor |
ASWATH, C
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Subject |
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Description |
Eggplant is an important vegetable in India and is grown throughout the tropical, sub tropical and warm temperate regions of the world. Bacterial wilt caused by Ralstonia solanacearum is a severe soil borne vascular disease commonly occurring in the warm, humid tropics. An investigation was conducted to identify molecular markers linked to bacterial wilt resistance in brinjal in the F2 mapping population developed by cross between a bacterial wilt resistant variety IIHR-7 with susceptible variety IIHR-108. 255 F2 plants were phenotyped by artificial root infection with Ralstonia solanacearum L. (3:1) ratio of resistancesusceptibility was observed in F2 and the genetic ratio indicated the resistance trait controlled by a single dominant gene. 39 EST-SSRs, 119 genomic SSRs and 100 RAPDs were used to genotype parents, F2 population though bulk segregant analysis (BSA). 29 genomic SSRs and 17 RAPDs had shown polymorphism in parental lines and were further used to screen the mapping population. No polymorphism was observed in the F2 population. Thus, no marker linked to bacterial wilt resistance in brinjal was identified in the present F2 population under study. Further, linkage analyses are to be performed to identify the genomic SSRs and RAPDs using more primers that are linked to the resistance trait in a larger population and integrated into a molecular marker assisted breeding programme of eggplant to breed bacterial wilt resistant varieties. |
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Date |
2016-12-19T10:52:25Z
2016-12-19T10:52:25Z 2011-08-09 |
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Type |
Thesis
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Identifier |
Th-10012
http://krishikosh.egranth.ac.in/handle/1/90914 |
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Language |
en
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Format |
application/pdf
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Publisher |
University of Agricultural Sciences GKVK, Bangalore
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