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FORMULIZATION AND DETERMINATION OF EFFECTIVENESS OF LIQUID INOCULANTS OF PLANT GROWTH PROMOTING RHIZOBACTERIA

KrishiKosh

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Title FORMULIZATION AND DETERMINATION OF EFFECTIVENESS OF LIQUID INOCULANTS OF PLANT GROWTH PROMOTING RHIZOBACTERIA
 
Creator DAYAMANI, K. J.
 
Contributor Manjunath, A.
 
Subject null
 
Description Laboratory investigations were conducted to identify suitable form and to
determine the suitable dose of application and time of addition of osmolytes for liquid
inoculant formulations of plant growth promoting rhizobacteria (PGPR) viz., Azotobacter
sp., Azospirillum sp., Acinetobacter sp., Bacillus sp. and Pseudomonas sp.
Osmolytes such as four grades of polyvinyl pyrrollidone (PVP), four grades of
polyethylene glycol (PEG), and glycerol were added to broth at three different
concentrations (0.5%, 1.0% and 2.0%). Supplementing the specific media with 2 %
Glycerol for Azotobacter sp., 2 % PVP K-15 for Bacillus sp., 1 % PEG 400 for
Azospirillum sp., 2 % PVP K-15 for Pseudomonas sp., and 2 % PEG 4000 for
Acinetobacter sp. resulted in the highest population densities. The addition of osmolytes
before inoculation was better than their addition at stationary growth phase. Survival
studies of these PGPR up to 180 days of storage revealed that the addition of glycerol
along with PVP and PEG sustains the highest population density of PGPR.
Greenhouse investigation was conducted to evaluate the effectiveness of liquid
inoculants using aerobic rice as an indicator plant. The highest chlorophyll concentration
was noticed in plants grown in soil amended with P in a soluble form (KH2PO4). In
general, inoculation of soil with either liquid or lignite formulations of all these PGPR
increased nitrogen concentration marginally, irrespective of type of nutrient amendment.
The addition of P in the form of potassium phosphate resulted in production of maximum
number of tillers. Biomass produced by plants grown in soil amended with potassium
phosphate was the highest. Although, biomass produced by plants grown in soil
inoculated with lignite as well as liquid formulations were higher than uninoculated
control, the differences were not statistically different.
 
Date 2017-02-27T06:15:12Z
2017-02-27T06:15:12Z
2010-08-16
 
Type Thesis
 
Identifier Th-9573
http://krishikosh.egranth.ac.in/handle/1/5810002664
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences GKVK, Bangalore