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e DNA - A New Concept in Fisheries Research In: ICAR Sponsored Winter School on Recent Advances in Fishery Biology Techniques for Biodiversity Evaluation and Conservation, 1-21 December 2018, Kochi.

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Relation http://eprints.cmfri.org.in/13309/
 
Title e DNA - A New Concept in Fisheries Research In: ICAR Sponsored Winter School on Recent Advances in Fishery Biology Techniques for Biodiversity Evaluation and Conservation, 1-21 December 2018, Kochi.
 
Creator Jayasankar, P
 
Subject Marine Fisheries
 
Description Environmental DNA (eDNA) is defined as the genetic material obtained from a water sample
containing no distinguishing signs of source macro-organisms. The method utilizes DNA which
is continuously excreted by organisms into the surrounding environment through mucus, gametes,
faeces, blood and other cells, and captures, analyses and obtains the nucleotide sequence of this
DNA based on an environmental sample. eDNA analysis has emerged as a potentially powerful tool
to access aquatic community structures. The inherent drawbacks in traditional approaches to monitor
fish biomass/abundance in exploited waters can be overcome by employing eDNA techniques.
Analysis of this eDNA can give us information on the organisms, their abundance and biomass
through two approaches – eDNA barcoding and eDNA metabarcoding. In the former, specific species
are targeted in samples using standard or quantitative PCR, and using traditional Sanger sequencing
method. In the latter, the whole community is screened using multiple conserved primers and Next
Gen Sequencing (NGS) is done. Studies suggest that eDNA metabarcoding outperforms traditional
survey methods in terms of non-invasive sampling, sensitivity and cost incurred. There is now
increased interest in using eDNA to supplement existing survey methods.
 
Date 2018
 
Type Teaching Resource
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/13309/1/5-Winter%20School%20on%20Recent%20Advances%20in%20Fishery%20Biology%20Techniques%20for%20Biodiversity%20Evaluation%20and%20Conservation_2018_Jayasankar%20P.pdf
Jayasankar, P (2018) e DNA - A New Concept in Fisheries Research In: ICAR Sponsored Winter School on Recent Advances in Fishery Biology Techniques for Biodiversity Evaluation and Conservation, 1-21 December 2018, Kochi. [Teaching Resource]