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In vitro Pearl Culture Techniques: A Biotechnological Approach

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Relation https://eprints.cmfri.org.in/13401/
 
Title In vitro Pearl Culture Techniques: A Biotechnological Approach
 
Creator Suja, C P
 
Subject Pearl oyster
CMFRI Special Publications
 
Description This book deals with the set up of tissue culture laboratory, protocols for maintaining a laboratory and methods for different experiments for nacre layer formation to achieve in vitro pearl formation. Indeed, this study explains the mantle tissue culture techniques of different pearl producing molluscs, cell proliferation with pearl sac formation and crystal deposition which form the basis for in vitro pearl formation. The techniques and methodologies used for pearl sac formation are unique and achieved after several trials and errors. Studies with different species of pearl producing molluscs reveal that, all the species are capable of producing cells, pearl sac and nacre crystals by techniques used in this study. Moreover, results of this study revealed that the shell bead is the best material for crystal adhesion. Formation of nacre layers is proved by Raman spectroscopy, Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Spectroscopy (EDS) in all the experimental shell beads. It is the first authenticated report of nacre layer formation over the shell bead in in vitro conditions with mantle cells of different pearl producing molluscs such as Pinctada fucata, Haliotis varia and Pteria avicular. This study is the stepping stone and needs further research to produce more lustrous pearls for commercialization.
 
Publisher ICAR - Central Marine Fisheries Research Institute
 
Date 2019
 
Type Book
PeerReviewed
 
Format text
 
Language en
 
Identifier https://eprints.cmfri.org.in/13401/1/CMFRI%20SP%20No.131_In%20vitro%20pearl%20culture%20technics_Eprints.pdf
Suja, C P (2019) In vitro Pearl Culture Techniques: A Biotechnological Approach. CMFRI Special Publication (131). ICAR - Central Marine Fisheries Research Institute, Kochi.