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Efficacy of 17 α‐methyl testosterone and letrozole on sex reversal of protogynous grouper, Epinephelus tauvina (Forskal, 1775) and spawning performance of sex‐reversed males

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Relation http://eprints.cmfri.org.in/13406/
https://onlinelibrary.wiley.com/doi/full/10.1111/are.12360
https://doi.org/10.1111/are.12360
 
Title Efficacy of 17 α‐methyl testosterone and letrozole on sex reversal of protogynous grouper, Epinephelus tauvina (Forskal, 1775) and spawning performance of sex‐reversed males
 
Creator Ranjan, Ritesh
Xavier, Biji
Dash, Biswajit
Edward, Loveson
Relangi, Suresh D
Pilli, Suresh Kumar
 
Subject Biochemical Study
Fish breeding
Fish Biotechnology
Groupers
 
Description This study aimed to test the efficacy of 17 α‐methyl testosterone (17 α‐MT) alone and in combination with letrozole, an aromatase inhibitor, for the induction of sex reversal in protogynous greasy grouper, Epinephelus tauvina. Further, the long‐lasting effects of these treatments and spawning performance of sex‐reversed males were also investigated. Greasy grouper with oocytes in the perinucleolus stage were implanted with 5 mg 17 α‐MT kg−1 body weight (T1), 5 mg 17 α‐MT and 0.2 mg letrozole kg−1 body weight (T2) and 5 mg 17 α‐MT with 0.4 mg letrozole kg−1 body weight (T3) and no androgens/enzyme inhibitor implanted (C). The 17 α‐MT alone and in combination of letrozole‐induced sex reversal in greasy grouper, whereas untreated control fish (C) showed normal ovarian development. However, T2 and T3 group showed 100% sex reversal and completion of spermatogenesis up to functional male phase in 2 and 3 months, respectively, whereas T1 group resulted in only 66.67% functional male with motile spermatozoa after 4 months. Sex‐reversed males successfully fertilized the eggs during induced spawning. There were significant differences on fertilization and hatching rates between T2 group (79.00 ± 4.36%; 77.67 ± 2.87%, respectively) and T1 group (57.67 ± 3.17%; 63.87 ± 2.91%, respectively). The result suggested that 17 α‐MT (5.0 mg kg−1 BW) in combination with letrozole (0.2 mg kg−1 BW) has the potential to produce 100% sex‐reversed male in short period in greasy grouper, which might greatly help in seed production of greasy grouper.
 
Date 2015
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/13406/1/Aquaculture%20Research_Ritesh%20Ranjan_2015_Protogynous%20grouper.pdf
Ranjan, Ritesh and Xavier, Biji and Dash, Biswajit and Edward, Loveson and Relangi, Suresh D and Pilli, Suresh Kumar (2015) Efficacy of 17 α‐methyl testosterone and letrozole on sex reversal of protogynous grouper, Epinephelus tauvina (Forskal, 1775) and spawning performance of sex‐reversed males. Aquaculture Research, 46. pp. 2065-2072.