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Biodegradation of methylene blue dye in a batch and continuous mode using biochar as packing media

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Title Biodegradation of methylene blue dye in a batch and continuous mode using
biochar as packing media
 
Creator Bharti, Vikash
Vikrant, Kumar
Goswami, Mandavi
Tiwari, Himanshu
Sonwani, Ravi Kumar
Lee, Jechan
Tsang, Daniel C.W.
Kim, Ki-Hyun
Saeed, Mohd
Kumar, Sunil
Rai, Birendra Nath
Giri, Balendu Shekher
Singh, Ram Sharan
 
Subject Industrial Wastewater Treatment & Disposal
Solid and Hazardous Wastes
 
Description Bacterial species for metabolizing dye molecules were isolated from dye rich water bodies. The best microbial
species for such an application was selected amongst the isolated bacterial populations by conducting methylene
blue (MB) batch degradation studies with the bacterial strains using NaCl-yeast as a nutrient medium. The most
suitable bacterial species was Alcaligenes faecalis (A. faecalis) according to 16S rDNA sequencing. Process
parameters were optimized and under the optimum conditions (e.g., inoculum size of 3 mL, temperature of 30 °C,
150 ppm, and time of 5 days), 96.2% of MB was removed. Furthermore, the effectiveness for the separation of
MB combining bio-film with biochar was measured by a bio-sorption method in a packed bed bioreactor (PBBR)
in which microbes was immobilized. The maximum MB removal efficiencies, when tested with 50 ppm dye using
batch reactors containing free A. faecalis cells and the same cells immobilized on the biochar surface, were found
to be 81.5% and 89.1%, respectively. The PBBR operated in continuous recycle mode at high dye concentration
of 500 ppm provided 87.0% removal of MB through second-order kinetics over 10 days. The % removal was
found in the order of PBBR>Immobilized batch>Free cell. The standalone biochar batch adsorption of MB can
be described well by the pseudo-second order kinetics (R2 ≥0.978), indicating the major contribution of
electron exchange-based valence forces in the sorption of MB onto the biochar surface. The Langmuir isotherm
suggested a maximum monolayer adsorption capacity of 4.69 mg g−1 at 40 °C which was very close to experimentally
calculated value (4.97 mg g−1). Moreover, the Casuarina seed biochar was reusable 5 times.
 
Publisher Elsevier
 
Date 2019
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/1170/1/Bhartiel.2019_ER.pdf
Bharti, Vikash and Vikrant, Kumar and Goswami, Mandavi and Tiwari, Himanshu and Sonwani, Ravi Kumar and Lee, Jechan and Tsang, Daniel C.W. and Kim, Ki-Hyun and Saeed, Mohd and Kumar, Sunil and Rai, Birendra Nath and Giri, Balendu Shekher and Singh, Ram Sharan (2019) Biodegradation of methylene blue dye in a batch and continuous mode using biochar as packing media. Environmental Research, 171. pp. 356-364. ISSN 0013-9351, ESSN: 1096-0953
 
Relation http://www.elsevier.com/locate/envres
http://neeri.csircentral.net/1170/