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Genomically Defined Paenibacillus polymyxa ND24 for Efficient Cellulase Production Utilizing Sugarcane Bagasse as a Substrate

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Title Genomically Defined Paenibacillus polymyxa ND24
for Efficient Cellulase Production Utilizing Sugarcane
Bagasse as a Substrate
 
Creator Bohra, Varsha
Tikariha, Hitesh
Dafale, Nishant A.
 
Subject Microbiology
 
Description Cellulolytic bacteria from cattle rumen with ability to hydrolyze cellulose rich
biomass were explored. The study selected Paenibacillus polymyxa ND24 from 847 isolates
as the most potent strain, which can efficiently produce cellulase by utilizing sugarcane
bagasse, rice straw, corn starch, CMC, and avicel as a sole carbon source. On annotation of
P. polymyxa ND24 genome, 116 members of glycoside hydrolase (GH) family from CAZy
clusters were identified and the presence of 10 potential cellulases was validated using protein
folding information. Cellulase production was further demonstrated at lab-scale 5-L bioreactor
exhibiting maximum endoglucanase activity up to 0.72 U/mL when cultivated in the medium
containing bagasse (2% w/v) after 72 h. The bagasse hydrolysate so produced was further
utilized for efficient biogas production. The presence of diverse hydrolytic enzymes and
formidable cellulase activity supports the use of P. polymyxa ND24 for cost-effective
bioprocessing of cellulosic biomass.
 
Publisher Springer
 
Date 2019
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/1171/1/Bohra2019_Article_GenomicallyDefinedPaenibacillu.pdf
Bohra, Varsha and Tikariha, Hitesh and Dafale, Nishant A. (2019) Genomically Defined Paenibacillus polymyxa ND24 for Efficient Cellulase Production Utilizing Sugarcane Bagasse as a Substrate. Applied Biochemistry and Biotechnology, 187 (1). pp. 266-281. ISSN 0273-2289, ESSN: 1559-0291
 
Relation https://link.springer.com/journal/12010
http://neeri.csircentral.net/1171/