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Growth associated degradation of aliphatic-aromatic copolyesters by Cryptococcus sp. MTCC 5455

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Title Growth associated degradation of aliphatic-aromatic copolyesters by
Cryptococcus sp. MTCC 5455
 
Creator Aarthy, Mayilvahanan
Puhazhselvan, Puhazhendi
Aparna, Ramchary
George, Antony Sebastian
Gowthaman, Marichetti Kuppuswami
Ayyadurai, Niraikulam
Masaki, Kazuo
Nakajima-Kambe, Toshiaki
Kamini, Numbi Ramudu
 
Subject Chemistry, Chemical Technology
 
Description The degradation of aliphatic-aromatic copolyesters, poly[(butylene succinate/terephthalate/isophthalate)-
co-(lactate)] (PBSTIL) and poly(butylene adipate co-terephthalate) (PBAT) under mesophilic
conditions was investigated using the yeast, Cryptococcus sp. MTCC 5455. Complete degradation of
PBSTIL and PBAT films was observed at 25 �C within 96 and 216 h, respectively. Lipase was produced
during the course of degradation and the encoding gene was expressed in Escherichia coli BL21 (DE3). The
production of lipase was enhanced by cumulative induction with 0.1mM IPTG and 1.5mM lactose for
24 h at 25 �C, which resulted in a maximum lipase activity of 27.75 ± 1.0 U/mL. Spectral studies of the
degraded polymeric films confirmed the hydrolysis of ester bonds by the lipase. Scanning electron microscopy
revealed the formation of cracks and holes on the surface of the polymeric films during
degradation. The results indicated the feasibility of the process towards degradation of polymeric waste
at ambient temperatures within a short period of time.
 
Publisher Elsevier
 
Date 2018
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/1178/1/10.1016%40j.polymdegradstab.2018.03.021.pdf
Aarthy, Mayilvahanan and Puhazhselvan, Puhazhendi and Aparna, Ramchary and George, Antony Sebastian and Gowthaman, Marichetti Kuppuswami and Ayyadurai, Niraikulam and Masaki, Kazuo and Nakajima-Kambe, Toshiaki and Kamini, Numbi Ramudu (2018) Growth associated degradation of aliphatic-aromatic copolyesters by Cryptococcus sp. MTCC 5455. Polymer Degradation and Stability, 152. pp. 20-28. ISSN 0141-3910, ESSN: 1873-2321
 
Relation http://www.elsevier.com/locate/polydegstab
http://neeri.csircentral.net/1178/