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Pectin induced transcriptome of a Rhizoctonia solani strain causing sheath blight disease in rice reveals insights on key genes and RNAi machinery for development of pathogen derived resistance

OAR@ICRISAT

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Relation http://oar.icrisat.org/11088/
http://dx.doi.org/10.1007/s11103-019-00843-9
10.1007/s11103-019-00843-9
 
Title Pectin induced transcriptome of a Rhizoctonia solani strain causing sheath blight disease in rice reveals insights on key genes and RNAi machinery for development of pathogen derived resistance
 
Creator Rao, T B
Chopperla, R
Methre, R
Punniakotti, E
Venkatesh, V
Sailaja, B
Reddy, M R
Yugander, A
Laha, G S
Madhav, M S
Sundaram, R M
Ladhalakshmi, D
Balachandran, S M
Mangrauthia, S K
 
Subject Rice
Genetics and Genomics
Plant Disease
 
Description Key message
RNAi mediated silencing of pectin degrading enzyme of R. solani gives a high level of resistance against sheath blight disease of rice.

Abstract
Rice sheath blight disease caused by Rhizoctonia solani Kuhn (telemorph; Thanatephorus cucumeris) is one of the most devastating fungal diseases which cause severe loss to rice grain production. In the absence of resistant cultivars, the disease is currently managed through fungicides which add to environmental pollution. To explore the potential of utilizing RNA interference (RNAi)-mediated resistance against sheath blight disease, we identified genes encoding proteins and enzymes involved in the RNAi pathway in this fungal pathogen. The RNAi target genes were deciphered by RNAseq analysis of a highly virulent strain of the R. solani grown in pectin medium. Additionally, pectin metabolism associated genes of R. solani were analyzed through transcriptome sequencing of infected rice tissues obtained from six diverse rice cultivars. One of the key candidate gene AG1IA_04727 encoding polygalacturonase (PG), which was observed to be significantly upregulated during infection, was targeted through RNAi to develop disease resistance. Stable expression of PG-RNAi construct in rice showed efficient silencing of AG1IA_04727 and suppression of sheath blight disease. This study highlights important information about the existence of RNAi machinery and key genes of R. solani which can be targeted through RNAi to develop pathogen-derived resistance, thus opening an alternative strategy for developing sheath blight-resistant rice cultivars.
 
Publisher Springer
 
Date 2019-02
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/11088/1/Pectin%20induced%20transcriptome.pdf
Rao, T B and Chopperla, R and Methre, R and Punniakotti, E and Venkatesh, V and Sailaja, B and Reddy, M R and Yugander, A and Laha, G S and Madhav, M S and Sundaram, R M and Ladhalakshmi, D and Balachandran, S M and Mangrauthia, S K (2019) Pectin induced transcriptome of a Rhizoctonia solani strain causing sheath blight disease in rice reveals insights on key genes and RNAi machinery for development of pathogen derived resistance. Plant Molecular Biology (TSI). pp. 1-13. ISSN 0167-4412