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Relevance of single-nucleotide polymorphism to the expression of resistin gene affecting serum and gingival crevicular fluid resistin levels in chronic periodontitis and type 2 diabetes mellitus: A randomized control clinical trial

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Title Relevance of single-nucleotide
polymorphism to the expression of
resistin gene affecting serum and
gingival crevicular fluid resistin levels
in chronic periodontitis and type 2
diabetes mellitus: A randomized
control clinical trial
 
Creator Rode, Pranita Avinash
Kolte, Rajashri Abhay
Kolte, Abhay Pandurang
Purohit, H. J.
Ahuja, Charu Ram
 
Subject Microbiology
 
Description Aim: The present study is aimed to investigate whether single‑nucleotide polymorphism (SNP) of resistin gene
(RETN) at −420 and +299 sites, is associated with resistin levels in serum and gingival crevicular fluid (GCF)
in periodontally healthy, chronic periodontitis (CP) with and without type 2 diabetes mellitus (T2DM) patients.
Materials and Methods: Serum and GCF samples were procured from all the 60 patients (twenty in each group)
of the three study groups i.e., periodontally healthy (Group I), CP (Group II) and CP with T2DM patients (Group III)
to analyze resistin levels using enzyme‑linked immunosorbent assay test and clinical parameters were assessed
at baseline and at 3 months after scaling and root planing (SRP). RETN polymorphism at −420 and +299 was
genotyped by polymerase chain reaction‑restriction fragment length polymorphism technique. Results: Patients
with SNP −420 and +299 were positively correlated with increased serum and GCF resistin levels in Group II and
Group III patients. SRP led to substantial reduction in the serum and GCF resistin levels. Conclusion: These
findings are suggestive of a biologic link between resistin, periodontal diseases, and periodontal diseases with
T2DM and RETN SNP at −420 and +299 in imparting increased resistin levels in inflammatory and diabetic
conditions.
 
Publisher Medknow Publications
 
Date 2019
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/1195/1/JIndianSocPeriodontol232131-4713969_011833.pdf
Rode, Pranita Avinash and Kolte, Rajashri Abhay and Kolte, Abhay Pandurang and Purohit, H. J. and Ahuja, Charu Ram (2019) Relevance of single-nucleotide polymorphism to the expression of resistin gene affecting serum and gingival crevicular fluid resistin levels in chronic periodontitis and type 2 diabetes mellitus: A randomized control clinical trial. Journal of Indian Society of Periodontology, 23 (2). pp. 131-136. ISSN 0972-124X, ESSN: 0975-1580
 
Relation http://www.jisponline.com/
http://neeri.csircentral.net/1195/