Record Details

Isolation and Enrichment of Bacteriophages by Membrane Filtration Immobilization Technique

IR@CSIR-NEERI

View Archive Info
 
 
Field Value
 
Title Isolation and Enrichment
of Bacteriophages by Membrane
Filtration Immobilization Technique
 
Creator Ghugare, Gaurav S.
Nimkande, Vijay D.
Khairnar, Krishna
 
Subject Microbiology
 
Description The method described here enables rapid bacteriophage isolation and enrichment
of host-specific bacteriophages from an environmental sample. This is
achieved by using a simple 0.45-μm Millipore membrane where a specific host
is immobilized on the membrane and a sample suspected of containing bacteriophages
is exposed to the immobilized cells with the help of a membrane
filtration unit. This filtration step facilitates host-specific interaction of bacteriophages
with the host and maximization of this interaction using a classic
membrane filtration method. Under the effect of vacuum from a vacuum pump,
a filter assembly provides a chance for every bacteriophage in the sample to
interact with the specific host on the membrane filter. Our technique allows
retaining specific bacteriophages on the membrane along with its host cells via
adsorption; these adsorbed bacteriophages (along with their hosts) on a filter
disc are then enriched in regular nutritive broth, tryptone soya broth (TSB),
by incubation. With help of a plaque assay method, host-specific phages of
various bacterial species can be isolated, segregated, and enriched. C � 2018 by
John Wiley & Sons, Inc.
 
Publisher Wiley
 
Date 2018
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/1198/1/Ghugare_et_al-2018-Current_Protocols_in_Cell_Biology.pdf
Ghugare, Gaurav S. and Nimkande, Vijay D. and Khairnar, Krishna (2018) Isolation and Enrichment of Bacteriophages by Membrane Filtration Immobilization Technique. Current Protocols in Cell Biology, 79 (1). pp. 1-8. ISSN 1934-2616
 
Relation https://currentprotocols.onlinelibrary.wiley.com/journal/19342616/
http://neeri.csircentral.net/1198/