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<p class="TitleMain" style="text-align: justify;">Extraction and rapid quantification of pure bixin from <em>Bixa orellena </em>L.</p>

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Title Statement <p class="TitleMain" style="text-align: justify;">Extraction and rapid quantification of pure bixin from <em>Bixa orellena </em>L.</p>
 
Added Entry - Uncontrolled Name Pattanaik, Lopa ; Center for Rural Development Technology, Indian Institute of Technology, New Delhi-110016, Delhi, India
Pradhan, Subhalaxmi ; Division of Chemistry, School of Basic and Applied Sciences, Galgotias University, Gautam Budha Nagar, U.P. 201308, India
Shaoo, Narendra Kumar; Center for Rural Development Technology, Indian Institute of Technology, New Delhi-110016, Delhi, India
Naik, Satya Narayan; Center for Rural Development Technology, Indian Institute of Technology, New Delhi-110016, Delhi, India
 
Uncontrolled Index Term Natural product extraction
Bixin; HPLC; Preparative TLC; Solvent extraction; Spectrophotometer.
 
Summary, etc. <p class="Abstract" style="text-align: justify;">Bixin (9Z-6, 6’-diapocarotene-6, 6’-dioate), one of the carotenoid-based colour extracted from the seeds of the tropical tree <em>Bixa orellana </em>L<em>.</em>, used primarily as a natural colouring agent in the food industry. In the present study, different solvents were used in both hot and cold conditions to extract bixin from <em>B. orellana </em>seeds, collected from two different geographical locations (Chhattisgarh and Uttaranchal) of India. The crude annatto extract was characterized by Thin Layer Chromatography (TLC). The major component, bixin was identified by TLC and further isolated from the crude extract by Preparative TLC (PTLC) method with a purity of 80%. Analysis of purified bixin contents isolated from samples was quantified by both High-Performance Liquid Chromatography (HPLC) and spectrophotometer. The results obtained from both the analytical techniques were comparable and lead to the conclusion that the highest amount of bixin was obtained from Chhattisgarh variety of annatto seed (21.13%), extracted by hot extraction method using acetone as a solvent. As compared to HPLC, the spectrophotometric analysis is an easy, rapid and cost-effective alternate route for the quantitative determination of isolated and purified bixin.<strong></strong></p>
 
Publication, Distribution, Etc. Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]
2019-03-25 13:30:00
 
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http://op.niscair.res.in/index.php/IJNPR/article/view/14167
 
Data Source Entry Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]; ##issue.vol## 9, ##issue.no## 4 (2018): December, 2018
 
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Nonspecific Relationship Entry http://op.niscair.res.in/index.php/IJNPR/article/download/14167/41866
http://op.niscair.res.in/index.php/IJNPR/article/download/14167/41867
http://op.niscair.res.in/index.php/IJNPR/article/download/14167/41868
http://op.niscair.res.in/index.php/IJNPR/article/download/14167/41869
http://op.niscair.res.in/index.php/IJNPR/article/download/14167/41870
http://op.niscair.res.in/index.php/IJNPR/article/download/14167/41871
http://op.niscair.res.in/index.php/IJNPR/article/download/14167/41872
http://op.niscair.res.in/index.php/IJNPR/article/download/14167/41873
 
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