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Manganese-Induced Neurotoxicity and Alterations in Gene Expression in Human Neuroblastoma SH-SY5Y Cells

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Title Manganese-Induced Neurotoxicity and Alterations in Gene
Expression in Human Neuroblastoma SH-SY5Y Cells
 
Creator Gandhi, Deepa
Sivanesan, Saravanadevi
Kannan, Krishnamurthi
 
Subject Microbiology
 
Description Manganese (Mn) is an essential trace element required
for many physiological functions including proper biochemical
and cellular functioning of the central nervous system
(CNS). However, exposure to excess level of Mn through
occupational settings or from environmental sources has been
associated with neurotoxicity. The cellular and molecular
mechanism of Mn-induced neurotoxicity remains unclear. In
the current study, we investigated the effects of 30-day exposure
to a sub-lethal concentration of Mn (100 μM) in human
neuroblastoma cells (SH-SY5Y) using transcriptomic approach.
Microarray analysis revealed differential expression
of 1057 transcripts in Mn-exposed SH-SY5Y cells as compared
to control cells. Gene functional annotation cluster analysis
exhibited that the differentially expressed genes were associated
with several biological pathways. Specifically, genes
involved in neuronal pathways including neuron differentiation
and development, regulation of neurogenesis, synaptic
transmission, and neuronal cell death (apoptosis) were found
to be significantly altered. KEGG pathway analysis showed
upregulation of p53 signaling pathways and neuroactive
ligand-receptor interaction pathways, and downregulation of
neurotrophin signaling pathway. On the basis of the gene expression
profile, possible molecular mechanisms underlying
Mn-induced neuronal toxicity were predicted.
 
Publisher Springer
 
Date 2018
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/1215/1/Gandhi2018_Article_Manganese-InducedNeurotoxicity.pdf
Gandhi, Deepa and Sivanesan, Saravanadevi and Kannan, Krishnamurthi (2018) Manganese-Induced Neurotoxicity and Alterations in Gene Expression in Human Neuroblastoma SH-SY5Y Cells. Biological Trace Element Research, 183 (2). pp. 245-253. ISSN 0163-4984, ESSN: 1559-0720
 
Relation https://link.springer.com/journal/12011
http://neeri.csircentral.net/1215/