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Genome Annotation and Validation of Keratin-Hydrolyzing Proteolytic Enzymes from Serratia marcescens EGD-HP20

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Field Value
 
Title Genome Annotation and Validation
of Keratin-Hydrolyzing Proteolytic Enzymes
from Serratia marcescens EGD-HP20
 
Creator Fuke, Priya
Gujar, Vaibhav V.
Khardenavis, A. A.
 
Subject Microbiology
 
Description Stabilization and utilization of poultry waste demand efficient biodegradation either
by mixture of enzymes or by microbial system that can produce different types of proteinhydrolyzing
enzymes. For utilization of this keratinous biomass, in the present study, genome
was sequenced and annotated for a bacterium having multiple enzymatic options for hydrolysis
of different soluble and insoluble protein fractions of poultry waste. Among the soluble
protein substrates, optimum production of enzyme and soluble protein was observed in case of
casein, whereas among the insoluble protein substrates, maximum production of enzyme was
achieved when broken nails were used. Conditions for enhanced enzyme activity with
concurrent degradation of keratin-rich poultry feather waste to protein-rich hydrolysate were
optimized for different growth parameters. The bacterium grew well and highest protease
production occurred in 144 h at mesophilic temperature (30 °C) and alkaline condition (pH 8–
10) with enzyme activities of 134 and 168 U/mL, respectively.
 
Publisher Springer
 
Date 2018
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/1227/1/Lohiya%20Ji-1.pdf
Fuke, Priya and Gujar, Vaibhav V. and Khardenavis, A. A. (2018) Genome Annotation and Validation of Keratin-Hydrolyzing Proteolytic Enzymes from Serratia marcescens EGD-HP20. Applied Biochemistry and Biotechnology, 184 (3). pp. 970-986. ISSN 0273-2289, ESSN: 1559-0291
 
Relation https://link.springer.com/journal/12010
http://neeri.csircentral.net/1227/