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Phytochemical antimicrobial and genetic analysis of Acacia nilotica from different geographical regions of Himachal Pradesh North West Himalaya

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Title Phytochemical antimicrobial and genetic analysis of Acacia nilotica from different geographical regions of Himachal Pradesh North West Himalaya
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Contributor Chauhan Pankaj Kumar
 
Subject Acacia nilotica
genetic variability
phytochemicals
RAPD
 
Description newline viii
newlineABSTRACT
newlineThe present study is mainly focused on the effects of seasonal and geographical changes on the quality and quantity of secondary metabolites of Acacia nilotica. The leaf samples of A. nilotica were collected from the six districts of Himachal Pradesh in three different seasons, i.e. summer (March-June), monsoon (July-October) and winter (November-February). The results of the qualitative phytochemical analysis revealed the presence of phlobatanins, reducing sugars, tannins, flavonoids, glycosides and steroids in all the samples in different seasons. The maximum alkaloid content, i.e. 81.20±1.70% was observed in the samples collected from Mandi district during winter season and least alkaloids i.e. 5.30±0.60% were present in the samples of Una district collected in summer season.
newlineOverall, TPC (274.17±4.56 and#956;g GAE/g), TFC (146.90±3.04 and#956;g RU/g), total alkaloids (81.20±1.70 %), ascorbic acid content (1035.78±4.79 and#956;g ascorbate/g extract), catalase activity (999.79±4.79 units/mg protein) and GPx activity (888.04±4.79 units/mg protein) were found highest in samples collected from high altitude (1502 amsl) as compared to low altitude areas (369 amsl). The findings suggested that the percentage of phytochemicals showed significant variations seasonally as well as geographically. The ethanolic leaf extract of A. nilotica showed higher activities against Gram negative strains, i.e. E. coli and S. aureus as compared to the fungal strain. The maximum zone of inhibition (22 mm) was shown against E. coli by ethanolic leaf extract of A. nilotica L. (75mg/ml) in the samples of Bilaspur district. Extracts of A. nilotica were found almost ineffective against C. albicans except samples of Bilaspur district (15-16 mm).
newlineFor RAPD analysis, 12 oligonucleotide decamer primers were used to amplify the genomic DNA from six samples of A. nilotica. Out of these, 7 primers generated RAPD fingerprints that revealed, 68 bands, in which 61 bands were found to be polymorphic. The size of amplified fragments ranged from 100 to 1500 bp. The highest percentage of polymorphism i.e. 100% was shown by OPN-20, OPN-16 and OPA-Y04 primers, whereas the lowest percentage of polymorphism i.e. 40% was shown by OPA-02. The genetic variations of the plant did not seem responsible for the amount of the phytochemical variance. The present study concluded that the
newlineix
newlinegeographical variables like seasons, geographical conditions and altitude have shown correlation with the accumulation of secondary metabolites in A. nilotica.
newlineKeywords: Acacia nilotica, genetic variability, RAPD, phytochemicals
Summary and Conclusion p.,94-96;Recommendation and Future Direction p.,97; References p.,98-114; Appendices p.,115-122; Publication p., 123
 
Date 2018-10-01T10:36:54Z
2018-10-01T10:36:54Z
27/08/2012
2017
08/09/2017
 
Type Ph.D.
 
Identifier http://hdl.handle.net/10603/217886
 
Language English
 
Relation 196
 
Rights university
 
Format 123p.
29cm
DVD
 
Coverage Biotechnology
 
Publisher Solan
Shoolini University of Biotechnology and Management Sciences
Faculty Of Biotechnology
 
Source University