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Arsenite-oxidation performance of microbes from abandoned iron ore

IR@CSIR-NEERI

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Title Arsenite-oxidation performance of microbes from abandoned iron ore
 
Creator Thul, Sanjog
Nigam, Bhavna
Tiwari, Sarita
Pandey, R A
 
Subject Environmental Resources, Conservation
Environmental Biotechnology
 
Description Arsenite is considered to be more toxic than arsenate (an average of 100 times). It can be oxidized to arsenate by chemical reaction or microbiological interactions. The aim of the present study was to investigate the arsenite-oxidizing microbes that exist in iron ore mine in India. We cultured 13 morphologically distinct bacterial strains, among which, 6 strains could grow in high concentrations and shows the arsenite transforming abilities. Analysis of the amplified 16S rDNA gene sequences of the isolates revealed them to belong to alpha-, gamma- Proteobacteria and Firmicutes particularly in genera Paenibacillus, Pseudomonas, Ochrobactrum, Enterobacter and Bacillus. Further, qualitative silver nitrate screening assay and quantification by HPLC-ICP-MS analysis of these strains indicated the transformation of arsenite to arsenate. Moreover, the isolates were genetically analyzed for presence of arsenic arsenite transporter gene (arsB) that indicates the genetic ability of bacteria to tolerate the most toxic arsenic species.
 
Publisher CSIR-NISCAIR
 
Date 2019-01
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/1245/1/IJBT%20ArsOxidizer.pdf
Thul, Sanjog and Nigam, Bhavna and Tiwari, Sarita and Pandey, R A (2019) Arsenite-oxidation performance of microbes from abandoned iron ore. Indian Journal of Biotechnology , 18 (1). pp. 34-41. ISSN 0975-0967
 
Relation http://nopr.niscair.res.in/handle/123456789/47119
http://neeri.csircentral.net/1245/