Record Details

Synergistic method for extraction of high purity Allophycocyanin from dry biomass of Arthrospira platensis and utilization of spent biomass for recovery of carotenoids.

IR@CSIR-CFTRI

View Archive Info
 
 
Field Value
 
Relation http://ir.cftri.com/14134/
https://doi.org/10.1016/j.seppur.2019.05.064
 
Title Synergistic method for extraction of high purity Allophycocyanin from dry biomass
of Arthrospira platensis and utilization of spent biomass for recovery of
carotenoids.
 
Creator Hrishikesh, A. Tavanandi
Prasad, V.
Raghavarao, K. S. M. S.
 
Subject 14 Carotenoid Chemistry
 
Description Allophycocyanin (A-PC) and carotenoids are high value low volume compounds
present in Arthrospira platensis which are usually ignored and left un-extracted (50-
80%) in the spent biomass after extraction of C-Phycocyanin. In the present work,
process parameters such as incubation time, solid-liquid (S/L) ratio and
surfactant/enzyme concentration was standardized for surfactant and enzyme-assisted
extraction of A-PC. Enzyme-assisted method resulted in very high A-PC purity (1.19)
with a yield of 32.27 mg/g dry biomass (db). To improve the yield, enzyme-assisted
method was combined with ultrasonication which resulted in the highest yield of 44.08
mg/g (db) with a purity of 1.09 (the highest reported till date during primary extraction).
The spent biomass after Low-Humidity (LH) drying was used for the extraction of
carotenoids and the best conditions were 80% concentration of ethanol, 1:4 S/L ratio,
pH 7, 40 oC temperature and 1 h extraction time. LH drying was observed to be a
possible alternative to freeze drying for drying of spent biomass.
 
Date 2019
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14134/1/Separation%20and%20Purification%20Technology%202019.pdf
Hrishikesh, A. Tavanandi and Prasad, V. and Raghavarao, K. S. M. S. (2019) Synergistic method for extraction of high purity Allophycocyanin from dry biomass of Arthrospira platensis and utilization of spent biomass for recovery of carotenoids. Separation and Purification Technology, 225. pp. 97-111. ISSN 1383-5866