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Agrobacterium mediated gentic transformation of grount for nucleocapsid mediated resistance to bud necrosis virus disease

Shodhganga@INFLIBNET

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Title Agrobacterium mediated gentic transformation of grount for nucleocapsid mediated resistance to bud necrosis virus disease
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Contributor Radhakrishnan, T
 
Subject Microbiology
grount
nucleocapsid mediated resistance
bud necrosis virus disease
 
Description Peanut bud necrosis disease caused peanut bud necrosis virus (PBNV), an enveloped
newlineRNA virus is a very serious problem in peanut or groundnut (Arachis hypogaea L.)
newlineproduction. Thrips Frankliniella fusca and Frankliniella occidentalis, are the vectors
newlinefor the virus, present a difficult and complicated challenge from the perspective of
newlineboth epidemiology and disease management. Simply controlling the vector typically
newlinehas not resulted in control of bud necrosis. A genetic engineering approach is proved
newlineto be of great potential for resistance enhancement against PBNV. In the present study
newlinetransgenic peanut lines with resistance to PBNV were developed from de embryonated
newlinecotyledon and immature leaf explants of groundnut cultivars Kadiri-6 (K-6) and K-134
newline(Vemana) using Agrobacterium tumefaciens strain LBA 4404 harboring Nucleocapsid
newlineProtein gene of Bud necrosis virus driven by CaMV 35S promoter. From the cultivar
newlineK-6, 1612 shoots were regenerated from a total of 327 de-embryonated cotyledon
newlineexplants. The survival of rooted and hardened plants in glasshouse was 92% and final
newlinerecovery of putative transgenics was 42.4%. In case of cultivar K-134, 69 deembryonated
newlineexplants were co-cultured and 398 shoots were regenerated. Survival of
newlineplants in the glasshouse was 89% and the putative transgenic recovery was 27.6%. A
newlinetotal of 3822 shoot buds were regenerated from 697 immature leaf explants from the
newlineseeds of cultivar K-6 were developed. Survival of plants under glasshouse conditions
newlinewas 91% and a 13.9% of final recovery of putative transgenics was observed.
References given
 
Date 2014-02-18T06:54:16Z
2014-02-18T06:54:16Z
2014-02-18
n.d.
2009
n.d.
 
Type Ph.D.
 
Identifier http://hdl.handle.net/10603/15973
 
Language English US
 
Relation -
 
Rights university
 
Format 104p.
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None
 
Coverage Microbiology
 
Publisher Rajkot
Saurashtra University
Department of Microbiology
 
Source INFLIBNET