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<em>E. coli</em> AB 1157 susceptibility test, MTT assay on MCF-7 and HeLa cell lines of root and leaf fractions of <em>Viburnum</em> Linn. species

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Title Statement <em>E. coli</em> AB 1157 susceptibility test, MTT assay on MCF-7 and HeLa cell lines of root and leaf fractions of <em>Viburnum</em> Linn. species
 
Added Entry - Uncontrolled Name Ponnudurai, K
Prabhu, K
Rani, S Shobha
Murthy, M Srinivasa
 
Uncontrolled Index Term Cytotoxicity; HeLa; MTT assay; MCF-7; Susceptibility test; Viburnum
 
Summary, etc. The aim of this study was to evaluate mutagenic bacterial susceptibility and cytotoxic potency of Viburnum coriaceum<br />Blume and Viburnum erubescens Wall.ex DC in order to report the actual chemotherapeutic potentials of these two species.<br />The methanolic (80%) leaf and chloroform root extracts of Viburnum Linn. Species were tested for their bacterial strain<br />based cytotoxicity employing Agar diffusion method suing E. coli AB 1157 strain. Also, the MTT assay was carried out<br />employing MCF-7 breast cancer cell lines and HeLa cervical cell lines. It was started with IC50 value determination of the<br />selected test extracts from the results of bacterial strain based cytotoxicity. Upon increase in concentration up to 1000<br />μg/mL in agar diffusion cytotoxicity studies VCMLE, VEMLE and VCCRE had shown diameter of inhibition zone 10 mm,<br />9 mm and 10 mm respectively. Among other extracts, the VEMLE and VCCRE were selected to go ahead with anticancer<br />activity by MTT assay. The potentials of extracts through cytotoxicity mechanism had produced 30-40% protection against<br />cancer cell lines. It was concluded that VEMLE and VCCRE had produced the cytotoxic effect on E. coli AB 1157 strain.<br />and were selected for the cytotoxic studies. The effects exhibited by the selected extracts may be due to the presence<br />of diverse number of active constituents present in Viburnum Linn species also may be to the presence of<br />unreported constituents.<br /><br />
 
Publication, Distribution, Etc. Indian Journal of Traditional Knowledge (IJTK)
2019-07-18 15:58:02
 
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http://op.niscair.res.in/index.php/IJTK/article/view/26806
 
Data Source Entry Indian Journal of Traditional Knowledge (IJTK); ##issue.vol## 18, ##issue.no## 3 (2019): Indian Journal of Traditional Knowledge
 
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