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Sri Lankan Finger Millet (Elucine coracana) Variety ‘Raavana’ as Potential Probiotic Source

OAR@ICRISAT

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Relation http://oar.icrisat.org/11202/
 
Title Sri Lankan Finger Millet (Elucine coracana) Variety ‘Raavana’ as Potential Probiotic Source
 
Creator Divisekera, D M W D
Samarasekera, J K R R
Goonerathne, J
Hettiarachchi, C
Gopalakrishnan, S
 
Subject Finger Millet
 
Description This study aims to isolate and identify probiotic potential lactic acid bacteria from
fermented Sri Lankan finger millet variety "Raavana" and to investigate the probiotic
characteristics, in vitro safety and efficacy. A bacterial isolate with typical lactic acid bacterial
phenotypic and biochemical characteristics was isolated and identified. Partial sequence of the
16S rRNA gene of the Sri Lankan strain was deposited in the NCBI gene bank as Lactococcus
lactis subsp. lactis FM_19LAB and the accession number MF480428 was obtained. It did
not demonstrate hemolysis, DNase, gelatine hydrolysis activity as well as did not acquire
complete resistance to any of the antibiotics tested hence indicating the safety. Lactococcus
lactis subsp. lactis FM_19LAB had the capacity to tolerate different concentrations of acid,
bile, phenol, salt, simulated gastric juices and range of temperatures. Further it exhibited
anti-microbial, anticancer and anti-oxidant activities. Further, Lactococcus lactis subsp. lactis
FM_19LAB assimilated cholesterol and produced lactic acid during the fermentation.
 
Publisher Indian Books and Periodicals
 
Date 2019
 
Type Book Section
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11202/1/Evolving%20Industrial%20Biotechnology%20in%20Developing%20Countries.pdf
Divisekera, D M W D and Samarasekera, J K R R and Goonerathne, J and Hettiarachchi, C and Gopalakrishnan, S (2019) Sri Lankan Finger Millet (Elucine coracana) Variety ‘Raavana’ as Potential Probiotic Source. In: Evolving Industrial Biotechnology in Developing Countries. Indian Books and Periodicals, pp. 167-183. ISBN 9789388982016