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Rapid and sensitive colorimetric detection of pathogens based on silver-urease interactions.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Rapid and sensitive colorimetric detection of pathogens based on silver-urease interactions.
 
Creator Singh, Pargat
Kakkar, Saloni
Bharti, .
Kumar, Rajesh
Bhalla, Vijayender
 
Subject QR Microbiology
 
Description Herein, we demonstrate a facile and economic approach for colorimetric detection of microbial pathogens in drinking water, employing silver-urease interactions. In the presence of harmful pathogens, receptor coated silver nanoparticles (AgNPs) preferentially bind to the bacterial surface and urease catalytically elevates the pH of the solution, which is sensed by a pH responsive chromogenic dye. The assay demarcates bacterial contamination levels up to 102 cells mL-1 in a field-friendly method.
 
Publisher Royal Society of Chemistry
 
Date 2019-04-18
 
Type Article
PeerReviewed
 
Relation https://pubs.rsc.org/en/content/articlelanding/2019/CC/C9CC00225A#!divAbstract
http://crdd.osdd.net/open/2418/
 
Identifier Singh, Pargat and Kakkar, Saloni and Bharti, . and Kumar, Rajesh and Bhalla, Vijayender (2019) Rapid and sensitive colorimetric detection of pathogens based on silver-urease interactions. Chemical communications (Cambridge, England), 55 (33). pp. 4765-4768. ISSN 1364-548X