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Genetics of fertility restoration and molecular mapping of Rf gene in rice (Oryza sativa L.)

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Title Genetics of fertility restoration and molecular mapping of Rf gene in rice (Oryza sativa L.)
 
Creator Singh, Satyapal
 
Subject hybrids, fertilizers, heterosis, planting, rice, yields, crossing over, grain, genes, developmental stages
 
Description Genetics of fertility restoration and molecular mapping of Rf gene in rice (Oryza sativa L.)
By
SATYAPAL SINGH
ABSTRACT

A study entitled “Genetics of fertility restoration and molecular mapping of Rf gene in rice (Oryza sativa L.)” was conducted at Research cum Instructional Farm and R.H. Richaria research MAS Laboratory, Department of Genetics and Plant Breeding, College of Agriculture, Indira Gandhi Krishi Vishwavidyalaya, Raipur, Chhattisgarh during Kharif season 2012-13 to 2013-14 respectively, identification of maintainers and restorers, combining ability effects, magnitude of heterosis, inheritance of fertility restorer (Rf) gene and molecular mapping of Rf gene. The experimental material included three CMS lines viz., IR58025A, CRMS31A and CRMS32A and twenty testers viz., RRH-1, RRH-2, R1557-1317-1-580-1, Nagina-22, Tarun Bhog, R1659-3629-1-462-1, Mancha, R-1240-927-3-1056-1, Jaldubi, Adhamchini, MTU-1001, TOX 981-11-2-3, Chinikapoor, R1949-1196-2-1, IR73007-44-1-2-3, IR72164-352-2-5-5, IR72910-177-1-1-3, R1213-3, Mahsuri Sel-1 and HR-NPT-6-3 and their sixty F1s were generated in line x tester pattern. These hybrids and parents were raised in Completely Randomized Block Design (CRBD) with two replications.
Observations were recorded for the different quantitative traits viz., days to 50% flowering, plant height, number of tillers per plant, productive tillers per plant, panicle length, number of spikelets per panicle, number of fertile spikelets per panicle, number of sterile spikelets per panicle, spikelet fertility (%), pollen fertility (%), biological yield per plant, grain yield per plant, thousand grain weight and harvest index.
Analysis of variance revealed that variance due to the line x tester was significant for almost all the characters. This suggested that sufficient variability is present in materials used for present study. Combining ability analysis was carried out following line x tester analysis. The analysis revealed the predominance of non-additive gene action for almost all the characters. This suggests the possibility of exploiting heterosis in the materials.
Character wise estimation of GCA effect of lines revealed that IR58025A was identified as a good general combiner for plant height, fertile spikelet per panicle and number of spikelet per panicle. Among the testers, Chinikapoor, Jaldubi, and Tarun bhog were found to be good general combiner for number of spikelet per panicle, fertile spikelet per plant and other related traits.
Cross combination IR-58025A x R-1240-927-3-1056-1 had high magnitude of SCA effects followed by IR-58025A x IR72910-177-1-1-3, CRMS32A x IR72910-177-1-1-3, CRMS31A x R1557-1317-1-580-1, CRMS31A x HR-NPT-6-3 and CRMS31A x RRH-1 for grain yield per plant. Significant and positive SCA effects were observed in nine hybrid combinations for spikelet fertility in which CRMS31A x MTU-1001 gave the highest value.
The cross combinations namely CRMS32A x R1557-1317-1-580-1 was identified as potential maintainer, whereas the cross combinations IR58025A x IR72164-352-2-5-5, IR58025A x TOX-981-11-2-3, CRMS31A x IR72164-352-2-5-5, IR58025A x R1949-1196-2-1, CRMS31A x Chinikapoor, CRMS32A x Tarun Bhog, CRMS31A x Mahsuri, CRMS31A x MTU-1001 and IR58025A x HR-NPT-6-3 were considered as potential restorers based on pollen and spikelet fertility.
Inheritance study in F2 population consisting four population viz., IR58025A x TOX981-11-2-3, CRMS31A x IR72164-352-2-5-5, IR58025A x R1949-1196-2-1 and IR58025A x IR72164-352-2-5-5 revealed that, two populations viz. IR58025A x TOX981-11-2-3 and IR58025A x IR72164-352-2-5-5 were identified to be governed with dominant monogenic gene action (3:1) for fertility restoration. Whereas CRMS31A x IR72164-352-2-5-5 indicating complementary gene action (9:7) and IR58025A x R1949-1196-2-1 having no interaction for fertility restoration.
Molecular analysis conducted in F2:3 generation consisting of a cross viz., IR58025A x IR72164-352-2-5-5 shows that, out of 22 SSR markers used in the present study, 9 markers viz., RM490, RM228, RM1108, RM6100, RM589, RM7003, RM6344, RM244 and RM171 has been found polymorphic between parents of IR58025A x IR72164-352-2-5-5 population. SSR markers RM490, RM228, RM1108, RM6100 and RM589 were screened across the population IR58025A x IR72164-352-2-5-5 consisting 23 highly fertile and 23 highly sterile plants for detection of Rf gene. But none of the markers segregate according to the population. The existence of a polymorphism between sterile and fertile lines is not adequate to associate this polymorphism with the existence of a linkage between marker and the Rf gene, due to all markers are showed heterozygous bands mostly over the population; therefore no linkage has been detected in the population.


Date: 11.5.2015
Department of Genetics and Plant Breeding Dr. Deepak Sharma
Indira Gandhi Krishi Vishwavidyalaya, Raipur (C.G) Chairman
Advisory Committee
 
Date 2015-07-02T11:49:38Z
2015-07-02T11:49:38Z
2015-05-11
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/63910
 
Language en
 
Relation ;316
 
Publisher Indira Gandhi Krishi Vishwavidyalaya, Raipur