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GENETIC AND MOLECULAR ANALYSIS OF BROWN PLANT HOPPER RESISTANT GENE(S) IN RICE (Oryza sativa L.)

KrishiKosh

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Title GENETIC AND MOLECULAR ANALYSIS OF BROWN PLANT HOPPER RESISTANT GENE(S) IN RICE (Oryza sativa L.)
 
Creator Ekka, Ruth Elizabeth
Sarawgi, A.K.
Verulkar, S.B.
Rana, D.K.
Saxena, R.R.
 
Subject —
Molecular Analysis
Plant Hopper
Oryza sativa L.
 
Description The present research work was undertaken in the Department of Genetics
and Plant Breeding, College of Agriculture, Raipur with the objectives of identifying
and monitoring the functional resistance genes in Chhattisgarh, understanding the
mode of inheritance of resistance to brown plant hopper in some selected resistant
donors, knowing the allelic relationships among resistance genes present in selected
donors and studying genetic diversity at molecular level in selected lines.
The experimental materials consisted of (a) A set of 500 advanced breeding
lines including susceptible checks and resistant checks, which were screened against
brown plant hopper at Raipur during Kharif 2009. (b) The 100 selected genotypes
including brown plant hopper resistant advanced breeding lines, susceptible checks
and resistant checks, which were screened at Raipur during Kharif 2010 and 2011 to
monitor the resistance. (c) F1, F2 and F3 populations of 9 crosses attempted for
inheritance studies and F1 and F2 population of the 17 crosses attempted for allelic
studies were screened against brown plant hopper to ascertain the genetic ratios.
Screening was carried out in glass house following 0-9 scale of standard evaluation
system of IRRI and plants with score 0-3 were classified as resistant and those with
scores 5-9 as susceptible. (d) A total of thirty eight microsatellite (SSR) markers were
used to assess the extent of genetic diversity across the twenty seven genotypes.
Out of four hundred seventy seven advanced breeding lines seven lines viz.,
R1641-914-1-400-1, R1723-1411-1-355-1, R1630-1248-2-846-1, R1709-2320-1-
2445-1, R1711-2409-2-2492-1, R1723-1413-2-356-1 and R1473-521-249-1-1 showed
highly resistant reaction and fifty lines showed resistant reaction over the years. The
inheritance investigation uncovered that brown plant hopper resistance in R-RF-55,
R1243-1224-578-1 and R1723-1413-3-357-1 was controlled by a single dominant
gene, while a single recessive gene governed resistance in R1546-1328-1-90-1 and
two independent dominant genes governed resistance in R1600-1124-2-618-1.
The allelic studies revealed that gene for brown plant hopper resistance present in
R1723-1413-3-357-1 was allelic to Bph1 (MTU15). The gene for resistance in
R1519-773-3-581-1, R1677-3473-1-4301-1, R1470-347-136-1-1 and R1670-3267-1-
3920-1 was non-allelic to resistance gene bph5 (ARC10550) and bph7 (T12). The
resistance gene present in R1519-773-3-581-1 was different from Bph1 (MTU15) and
Bph6 (Swarnalata). Similarly resistance gene of R1677-3473-1-4301-1 was different
from bph2 (H105) and Bph6 (Swarnalata).
The twenty one SSR markers generated polymorphic patterns while seventeen
SSR markers found monomorphic. A total of 61 alleles were detected in 27 rice
genotypes and the number of alleles per locus ranged from 2 to 5 with an average of
2.9 per locus. Size differences between the smallest and largest alleles varied from
5 bp (approx) to 40 bp (approx). Polymorphism information content ranged from
0.14 to 0.72 with an average of 0.48. A dendogram based on UPGMA analysis
showed the genetic diversity among the 27 rice genotypes and similarity coefficients
ranged from 0.23 to 0.91. Analysis of clusters of dendogram revealed highest
91 per cent genetic similarity between genotypes Kariyabenikat K 1866 and
Danwar2511 in cluster II and also between genotypes Danteshwari and Samleshwari
in cluster I.
 
Date 2015-07-30T12:22:23Z
2015-07-30T12:22:23Z
2012-05
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/63941
 
Relation T-2776;149
 
Publisher Indira Gandhi Krishi Vishwavidyalaya, Raipur