Record Details

Integrated transcriptome, small RNA and degradome sequencing approaches provide insights into Ascochyta blight resistance in chickpea

OAR@ICRISAT

View Archive Info
 
 
Field Value
 
Relation http://oar.icrisat.org/11269/
https://doi.org/10.1111/pbi.13026
10.1111/pbi.13026
 
Title Integrated transcriptome, small RNA and degradome sequencing approaches provide insights into Ascochyta blight resistance in chickpea
 
Creator Garg, V
Khan, A W
Kudapa, H
Kale, S M
Chitikineni, A
Qiwei, S
Sharma, M
Li, C
Zhang, B
Xin, L
Kavi Kishor, P B
Varshney, R K
 
Subject Biotic Stress
Chickpea
Genetics and Genomics
 
Description Ascochyta blight (AB) is one of the major biotic stresses known to limit the chickpea production worldwide. To dissect the complex mechanisms of AB resistance in chickpea, three approaches,namely, transcriptome, small RNA and degradome sequencing were used. The transcriptome
sequencing of 20 samples including two resistant genotypes, two susceptible genotypes and one introgression line under control and stress conditions at two time points (3rd and 7th day post inoculation) identified a total of 6767 differentially expressed genes (DEGs). These DEGs were
mainly related to pathogenesis-related proteins, disease resistance genes like NBS-LRR, cell wall biosynthesis and various secondary metabolite synthesis genes. The small RNA sequencing of the samples resulted in the identification of 651 miRNAs which included 478 known and 173 novel
miRNAs. A total of 297 miRNAs were differentially expressed between different genotypes, conditions and time points. Using degradome sequencing and in silico approaches, 2131 targets were predicted for 629 miRNAs. The combined analysis of both small RNA and transcriptome
datasets identified 12 miRNA-mRNA interaction pairs that exhibited contrasting expression in resistant and susceptible genotypes and also, a subset of genes that might be posttranscriptionally silenced during AB infection. The comprehensive integrated analysis in the study provides better insights into the transcriptome dynamics and regulatory network components
associated with AB stress in chickpea and, also offers candidate genes for chickpea improvement.
 
Publisher Wiley
 
Date 2019-05
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11269/1/pbi.13026.pdf
Garg, V and Khan, A W and Kudapa, H and Kale, S M and Chitikineni, A and Qiwei, S and Sharma, M and Li, C and Zhang, B and Xin, L and Kavi Kishor, P B and Varshney, R K (2019) Integrated transcriptome, small RNA and degradome sequencing approaches provide insights into Ascochyta blight resistance in chickpea. Plant Biotechnology Journal (TSI), 17 (5). pp. 914-931. ISSN 1467-7644