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NorA efflux pump inhibitory activity of coumarins from Mesua ferrea.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title NorA efflux pump inhibitory activity of coumarins from Mesua ferrea.
 
Creator Roy, Somendu K
Kumari, Neela
Pahwa, Sonika
Agrahari, Udai C
Bhutani, Kamlesh K
Jachak, Sanjay M
Nandanwar, Hemraj
 
Subject QR Microbiology
 
Description The purpose of this investigation was to study the modulator and efflux pump inhibitor activity of coumarins isolated from Mesua ferrea against clinical strains as well as NorA-over expressed strain of Staphylococcus aureus 1199B. Seven coumarins were tested for modulator activity using ethidium bromide (EtBr) as a substrate. Compounds 1, 4-7 modulated the MIC of EtBr by ≥ 2 fold against wild type clinical strains of S. aureus 1199 and S. aureus 1199B, whereas compounds 4-7 modulated the MIC of EtBr by ≥ 16 fold against MRSA 831. Compounds 1, 4-7 also reduced the MIC of norfloxacin by ≥ 8 fold against S. aureus 1199B, and 4-6 reduced the MIC of norfloxacin by ≥ 8 fold against MRSA 831 at half of their MICs. Inhibition of EtBr efflux by NorA-overproducing S. aureus 1199B and MRSA 831 confirmed the role of compounds 4-6 as NorA efflux pump inhibitors (EPI). Dose-dependent activity at sub-inhibitory concentration (6.25 μg/mL) suggested that compounds 4 and 5 are promising EPI compared to verapamil against 1199B and MRSA 831 strains.
 
Publisher Elsevier Science
 
Date 2013-10
 
Type Article
PeerReviewed
 
Relation https://linkinghub.elsevier.com/retrieve/pii/S0367-326X(13)00186-X
http://crdd.osdd.net/open/2452/
 
Identifier Roy, Somendu K and Kumari, Neela and Pahwa, Sonika and Agrahari, Udai C and Bhutani, Kamlesh K and Jachak, Sanjay M and Nandanwar, Hemraj (2013) NorA efflux pump inhibitory activity of coumarins from Mesua ferrea. Fitoterapia, 90. pp. 140-50. ISSN 1873-6971