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Role of oxyRKP, a novel LysR-family transcriptional regulator, in antimicrobial resistance and virulence in Klebsiella pneumoniae.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Role of oxyRKP, a novel LysR-family transcriptional regulator, in antimicrobial resistance and virulence in Klebsiella pneumoniae.
 
Creator Srinivasan, Vijaya Bharathi
Mondal, Amitabha
Venkataramaiah, Manjunath
Chauhan, Neeraj Kumar
Rajamohan, Govindan
 
Subject QR Microbiology
 
Description Klebsiella pneumoniae is a Gram-negative bacillus that causes serious infections in immunocompromised human hosts and exhibits significant multidrug resistance. In this study, we identified a novel lysR-family regulator (designated oxyR(KP)) in the genome of K. pneumoniae NTUH-K2044 whose functions have remained enigmatic so far. Functional characterization of the putative lysR regulator oxyR(KP) with respect to cellular physiology and antimicrobial susceptibility was performed by generating an isogenic mutant, ΔoxyR(KP) in a hypervirulent clinical isolate of K. pneumoniae. The K. pneumoniae oxyR(KP) mutant was sensitive to hyperosmotic and bile conditions. Disruption of oxyR(KP) increased the susceptibility of K. pneumoniae to oxidative (0.78947 mM hydrogen peroxide) and nitrosative (30 mM acidified nitrite) stress by ~1.4-fold and ~10-fold, respectively. Loss of the Klebsiella regulator led to a decrease in the minimum inhibitory concentrations for chloramphenicol (10-fold), erythromycin (6-fold), nalidixic acid (>50-fold) and trimethoprim (10-fold), which could be restored following complementation. The relative change in expression of resistance-nodulation-cell division super family (RND) efflux gene acrB was decreased by approximately fivefold in the oxyR(KP) mutant as evidenced by qRT-PCR. In a Caenorhabditis elegans model, the oxyR(KP) mutant exhibited significantly (P
 
Publisher Microbiology Society
 
Date 2013-07-01
 
Type Article
PeerReviewed
 
Relation https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.065052-0#tab2
http://crdd.osdd.net/open/2456/
 
Identifier Srinivasan, Vijaya Bharathi and Mondal, Amitabha and Venkataramaiah, Manjunath and Chauhan, Neeraj Kumar and Rajamohan, Govindan (2013) Role of oxyRKP, a novel LysR-family transcriptional regulator, in antimicrobial resistance and virulence in Klebsiella pneumoniae. Microbiology (Reading, England), 159 (Pt 7). pp. 1301-14. ISSN 1465-2080