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Methodology: ssb-MASS: a single seed-based sampling strategy for marker-assisted selection in rice

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Relation http://oar.icrisat.org/11299/
https://doi.org/10.1186/s13007-019-0464-2
10.1186/s13007-019-0464-2
 
Title Methodology: ssb-MASS: a single seed-based sampling strategy for marker-assisted selection in rice
 
Creator Arbelaez, J D
Tandayu, E
Reveche, M Y
Jarana, A
van Rogen, P
Sandager, L
Stolt, P
Ng, E
Varshney, R K
Kretzschmar, T
Cobb, J
 
Subject Molecular Biology
Plant Breeding
Rice
Genetics and Genomics
 
Description Background Integrated breeding approaches such as combining marker-assisted selection and rapid line fixation through single-seed-descent, can effectively increase the frequency of desirable alleles in a breeding program and increase the rate of genetic gain for quantitative traits by shortening the breeding cycle. However, with most genotyping being outsourced to 3rd party service providers’ nowadays, sampling has become the bottleneck for many breeding programs. While seed-chipping as prevailed as an automatable seed sampling protocol in many species, the symmetry of rice seeds makes this solution as laborious and costly as sampling leaf tissue. The aim of this study is to develop, validate and deploy a single seed sampling strategy for marker-assisted selection of fixed lines in rice that is more efficient, cost-effective and convenient compared to leaf-based sampling protocols without compromising the accuracy of the marker-assisted selection results. Results Evaluations replicated across accessions and markers showed that a single rice seed is sufficient to generate enough DNA (7–8 ng/μL) to run at least ten PCR trait-markers suitable for marker-assisted selection strategies in rice. The DNA quantity and quality extracted from single seeds from fixed lines (F6) with different physical and/or chemical properties were not significantly different. Nor were there significant differences between single seeds collected 15 days after panicle initiation compared to those harvested at maturity. A large-scale comparison between single seed and leaf-based methodologies showed not only high levels of genotypic concordance between both protocols (~ 99%) but also higher SNP call rates in single seed (99.24% vs. 97.5% in leaf). A cost–benefit analysis showed that this single seed sampling strategy decreased the cost of sampling fourfold. An advantage of this approach is that desirable genotypes can be selected before investing in planting activities reducing the cost associated with field operations. Conclusion This study reports the development of a cost-effective and simple single seed genotyping strategy that facilitates the adoption and deployment of marker-assisted selection strategies in rice. This will allow breeders to increase the frequency of favorable alleles and combine rapid generation advancement techniques much more cost-effectively accelerating the process and efficiency of parental selection and varietal development.
 
Publisher BioMed Central Ltd
 
Date 2019-07
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11299/1/s13007-019-0464-2.pdf
Arbelaez, J D and Tandayu, E and Reveche, M Y and Jarana, A and van Rogen, P and Sandager, L and Stolt, P and Ng, E and Varshney, R K and Kretzschmar, T and Cobb, J (2019) Methodology: ssb-MASS: a single seed-based sampling strategy for marker-assisted selection in rice. Plant Methods (TSI), 15(1) (78). pp. 1-11. ISSN 1746-4811